| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD247 |
| Uniprot No | P20963 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 62-164aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSRVKFSRSADAPAYQQGQNQLYNELNLG RREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGM KGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD247(CD3ζ)重组蛋白的3篇参考文献示例(注：文献为假设性概括，实际引用请核实原文)：
---
1. **文献名称**：*Expression and functional characterization of recombinant CD3ζ in T cell signaling*
**作者**：Smith A, Jones B
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组CD247蛋白，验证其与TCR复合物其他亚基的结合能力，证实其在T细胞激活中的关键信号传导作用。
2. **文献名称**：*Structural insights into CD3ζ chain dimerization via recombinant protein analysis*
**作者**：Lee C, Garcia KC
**摘要**：通过重组CD247蛋白的晶体结构解析，揭示了其胞内结构域的二聚化机制，为优化CAR-T细胞中CD3ζ信号模块的设计提供依据。
3. **文献名称**：*Recombinant CD3ζ enhances antitumor activity in engineered NK cells*
**作者**：Wang Y, Sadelain M
**摘要**：利用重组CD247蛋白改造自然杀伤细胞(NK)，证明其与共刺激分子融合后可显著提升肿瘤细胞杀伤效能，推动新型免疫疗法开发。
---
**备注**：实际文献需通过PubMed或学术数据库检索关键词(如“CD247 recombinant”、“CD3ζ expression”)获取。部分相关领域权威作者包括：K. Christopher Garcia(结构生物学)、Michel Sadelain(CAR-T应用)等。
CD247. also known as CD3ζ (zeta chain), is a critical component of the T-cell receptor (TCR) complex, playing a central role in T-cell signaling and immune response regulation. As a transmembrane protein, it contains immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain, which are essential for propagating intracellular signals upon TCR engagement with antigen-presenting cells. Following antigen recognition, CD3ζ undergoes phosphorylation, initiating downstream signaling cascades that drive T-cell activation, cytokine production, and cytotoxic functions.
Recombinant CD247 protein is engineered in vitro using expression systems (e.g., mammalian cells, E. coli) to study its structural and functional properties. Purified via affinity chromatography, it retains key domains required for interactions with other TCR subunits (CD3ε, δ, γ) and signaling molecules. Researchers utilize this protein to dissect mechanisms of T-cell activation, immune tolerance, and pathologies like autoimmune diseases or T-cell dysfunction in cancer. Its ITAM motifs are particularly scrutinized for their role in modulating signal strength and immune synapse formation.
In therapeutic contexts, CD3ζ is a cornerstone of chimeric antigen receptor (CAR)-T cell engineering, where its signaling domain is fused with antigen-targeting moieties to redirect T-cell specificity against tumors. Studies also explore its involvement in checkpoint inhibitor resistance and as a biomarker for immune-related adverse events. By enabling precise biochemical and functional analyses, recombinant CD247 accelerates both basic immunology research and the development of next-generation immunotherapies.
×
