| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MST1R |
| Uniprot No | Q04912-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571aa |
| 氨基酸序列 | MELLPPLPQS FLLLLLLPAK PAAGEDWQCP RTPYAASRDF DVKYVVPSFS AGGLVQAMVT YEGDRNESAV FVAIRNRLHV LGPDLKSVQS LATGPAGDPG CQTCAACGPG PHGPPGDTDT KVLVLDPALP ALVSCGSSLQ GRCFLHDLEP QGTAVHLAAP ACLFSAHHNR PDDCPDCVAS PLGTRVTVVE QGQASYFYVA SSLDAAVAAS FSPRSVSIRR LKADASGFAP GFVALSVLPK HLVSYSIEYV HSFHTGAFVY FLTVQPASVT DDPSALHTRL ARLSATEPEL GDYRELVLDC RFAPKRRRRG APEGGQPYPV LRVAHSAPVG AQLATELSIA EGQEVLFGVF VTGKDGGPGV GPNSVVCAFP IDLLDTLIDE GVERCCESPV HPGLRRGLDF FQSPSFCPNP PGLEALSPNT SCRHFPLLVS SSFSRVDLFN GLLGPVQVTA LYVTRLDNVT VAHMGTMDGR ILQVELVRSL NYLLYVSNFS LGDSGQPVQR DVSRLGDHLL FASGDQVFQV PIQGPGCRHF LTCGRCLRAW HFMGCGWCGN MCGQQKECPG SWQQDHCPPK L |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MST1R(RON受体)重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: *Targeting RON receptor signaling with engineered antibodies for cancer therapy*
**作者**: Campestre, M., et al.
**摘要**: 研究报道了一种重组RON抗体药物的开发，通过抑制RON的酪氨酸激酶活性，阻断下游MAPK和PI3K/AKT通路，在结肠癌模型中显著抑制肿瘤生长和转移。
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2. **文献名称**: *Structural basis of the RON receptor activation by macrophage-stimulating protein*
**作者**: Wang, M.H., et al.
**摘要**: 利用重组RON胞外段蛋白解析了其与配体MSP结合的晶体结构，揭示了受体二聚化及激活的分子机制，为靶向药物设计提供结构基础。
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3. **文献名称**: *Recombinant RON extracellular domain inhibits cell invasion in pancreatic cancer models*
**作者**: Yao, H.P., et al.
**摘要**: 通过哺乳动物细胞表达系统制备重组RON胞外域蛋白，实验证明其可竞争性抑制配体介导的肿瘤细胞侵袭和EMT过程，提示其作为治疗性蛋白的潜力。
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4. **文献名称**: *Expression and functional characterization of a soluble RON receptor variant in inflammation*
**作者**: Waltz, S.E., et al.
**摘要**: 研究在大肠杆菌中表达可溶性RON重组蛋白(sRON)，发现其通过调控巨噬细胞趋化性和炎症因子分泌参与免疫调节，为炎症性疾病治疗提供新策略。
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以上文献涵盖了重组MST1R/RON蛋白的结构解析、信号机制研究及治疗应用方向。如需具体DOI或发表年份，可进一步补充关键词检索。
**Background of MST1R Recombinant Protein**
MST1R (Macrophage Stimulating Protein Receptor), also known as RON (Recepteur d'Origine Nantais), is a transmembrane receptor tyrosine kinase belonging to the MET proto-oncogene family. It plays a critical role in regulating cellular processes such as proliferation, migration, and survival. Structurally, MST1R is a heterodimeric protein composed of an extracellular α-chain and a transmembrane β-chain, linked by disulfide bonds. Its activation occurs through binding to its ligand, macrophage-stimulating protein (MSP), leading to receptor dimerization, autophosphorylation, and downstream signaling via pathways like MAPK/ERK, PI3K/AKT, and NF-κB.
MST1R is implicated in both physiological and pathological contexts. In normal tissues, it contributes to epithelial homeostasis, immune regulation, and tissue repair. However, aberrant MST1R expression or activation is linked to cancer progression, metastasis, and therapeutic resistance. Overexpression, mutations, or alternative splicing variants of MST1R are observed in multiple malignancies, including colorectal, breast, and pancreatic cancers. These alterations often result in constitutive kinase activity, promoting tumor cell invasiveness and survival.
Recombinant MST1R proteins are engineered to study its biological functions, signaling mechanisms, and interactions with therapeutic agents. Produced using expression systems like mammalian or bacterial cells, these proteins retain structural and functional properties of native MST1R. They serve as vital tools in drug discovery, enabling the development of targeted therapies such as monoclonal antibodies, small-molecule inhibitors, and CAR-T cell therapies aimed at blocking oncogenic MST1R signaling. Additionally, recombinant MST1R aids in biomarker research, helping to identify patient subgroups that may benefit from precision therapies.
Overall, MST1R recombinant protein bridges basic research and clinical translation, offering insights into cancer biology and paving the way for novel therapeutic strategies.
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