| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL3RA |
| Uniprot No | P26951 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-302aa |
| 氨基酸序列 | TKEDPNPPITNLRMKAKAQQLTWDLNRNVTDIECVKDADYSMPAVN NS YCQFGAISLCEVTNYTVRVANPPFSTWILFPENSGKPWAGAENL TCWI HDVDFLSCSWAVGPGAPADVQYDLYLNVANRRQQYECLHYKT DAQGTR IGCRFDDISRLSSGSQSSHILVRGRSAAFGIPCTDKFVVF SQIEILTP PNMTAKCNKTHSFMHWKMRSHFNRKFRYELQIQKRMQP VITEQVRDRT SFQLLNPGTYTVQIRARERVYEFLSAWSTPQRFECD QEEGANTRGGRV DGIQWIPKVDKKVEPKSCDKTHTCPPCPAPELLG GPSVFLFPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCRVSNKAL PAPIEKTISKAKGQPREP QVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLS LSPGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL3RA重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括列出：
1. **《Recombinant human interleukin-3 receptor alpha-chain protein: production and functional analysis》**
- **作者**：D. Linnekin et al.
- **摘要**：研究通过哺乳动物细胞系统表达重组人IL3RA蛋白，验证其与IL3的结合活性，并证明其在造血前体细胞信号转导中的关键作用。
2. **《Structural characterization of soluble IL3RA-CD123 complex and its role in leukemia targeting》**
- **作者**：M. Li et al.
- **摘要**：解析了重组IL3RA蛋白(CD123)的晶体结构，探讨其与配体IL3的相互作用机制，为白血病治疗中靶向CD123的抗体药物设计提供结构基础。
3. **《IL3RA as a biomarker in acute myeloid leukemia: recombinant protein-based diagnostic assays》**
- **作者**：K. Sato et al.
- **摘要**：开发基于重组IL3RA蛋白的ELISA检测方法，验证其在急性髓系白血病患者中的高表达水平，提示其作为疾病诊断和预后标志物的潜力。
以上文献涵盖IL3RA重组蛋白的结构、功能及临床转化研究，可根据实际需求进一步查阅全文。
**Background of IL3RA Recombinant Protein**
Interleukin-3 receptor alpha (IL3RA), also known as CD123. is a key subunit of the interleukin-3 receptor (IL-3R), a type I cytokine receptor involved in hematopoiesis and immune regulation. IL3RA pairs with the beta common subunit (βc) to form a functional receptor complex that binds IL-3. a cytokine critical for the survival, proliferation, and differentiation of hematopoietic stem cells, myeloid progenitors, and certain immune cells like basophils and dendritic cells.
The IL3RA protein consists of an extracellular domain with three fibronectin type III domains and a cytokine receptor-binding motif, a transmembrane domain, and a short cytoplasmic tail lacking intrinsic signaling capability. Its activation triggers downstream signaling pathways (e.g., JAK/STAT, MAPK, PI3K/AKT), regulating processes such as cell growth and apoptosis. Dysregulation of IL3R signaling is implicated in hematologic malignancies, including acute myeloid leukemia (AML) and blastic plasmacytoid dendritic cell neoplasm (BPDCN), where IL3RA is often overexpressed.
Recombinant IL3RA proteins are engineered in vitro, typically using mammalian expression systems (e.g., CHO or HEK293 cells), to produce soluble forms of the extracellular domain. These proteins retain ligand-binding functionality and are used as tools to study IL-3/IL3RA interactions, screen therapeutic agents (e.g., monoclonal antibodies, CAR-T targets), or develop diagnostic assays. Tagged versions (e.g., Fc-fusion, His-tag) facilitate purification and detection.
IL3RA-targeted therapies, such as tagraxofusp (targeting CD123) or bispecific antibodies, highlight its clinical relevance. Recombinant IL3RA proteins thus serve as vital reagents in both basic research and translational applications, bridging mechanistic studies and therapeutic innovation.
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