Mouse Monoclonal AcetylP53(K382)(15F4) Antibody

The acetyl p53 (K382) (15F4) antibody is a monoclonal antibody specifically designed to detect p53 protein acetylated at lysine 382 (K382), a post-translational modification critical for regulating p53 activity. p53. a tumor suppressor protein, plays a central role in maintaining genomic stability by inducing cell cycle arrest, DNA repair, or apoptosis in response to cellular stress. Acetylation at K382 (mediated by histone acetyltransferases like PCAF or CBP/p300) enhances p53's transcriptional activity by promoting its binding to target gene promoters, such as those involved in apoptosis (e.g., BAX, PUMA) or cell cycle regulation (e.g., p21). The 15F4 clone recognizes this specific acetylated epitope and is widely used in applications like Western blotting, immunoprecipitation, and immunofluorescence to study p53 activation under conditions of DNA damage, oxidative stress, or chemotherapeutic treatments. Its specificity makes it valuable for investigating p53-dependent pathways in cancer biology, therapeutic response studies, and epigenetic regulation. Researchers often employ this antibody to explore correlations between p53 acetylation status, tumor progression, and patient prognosis, particularly in cancers with p53 mutations or dysregulated acetylation pathways.