Mouse Monoclonal α-tubulin(AcetylLys40)(4A8)(4A8) Antibody

The α-tubulin (Acetyl Lys40) (4A8) antibody is a monoclonal antibody specifically targeting acetylated lysine 40 (K40) on α-tubulin, a post-translational modification critical for microtubule stability and function. Acetylation at K40 occurs within the luminal domain of microtubules, mediated by α-tubulin acetyltransferase (αTAT1), and is associated with long-lived, stable microtubule structures in cilia, flagella, and axons. This modification reduces microtubule rigidity, influencing intracellular transport, cell motility, and cytoskeletal organization. The 4A8 clone is widely used in research to distinguish acetylated α-tubulin from its non-acetylated form, enabling studies on microtubule dynamics in processes like cell division, differentiation, and neuronal development. Validated for applications including Western blotting, immunofluorescence, and immunohistochemistry, this antibody is particularly valuable in neurobiology, ciliopathy research, and cancer studies, where altered microtubule stability is linked to disease mechanisms. Its specificity for the conserved K40 acetylation site across species (e.g., human, mouse, rat) ensures broad experimental utility. Discoveries using this antibody have advanced understanding of how microtubule post-translational modifications regulate cellular architecture and signaling pathways.