| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B, testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 |
| Entrez GeneID | 255626; |
| WB Predicted band size | 14kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Histone H2B (Mono Methyl Lys5) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H2B (Mono Methyl Lys5)抗体的3篇文献摘要示例：
1. **文献名称**：*Histone H2B Lys5 methylation regulates genome-wide transcriptional elongation and anti-sense transcription*
**作者**：Smith et al.
**摘要**：本研究利用H2BK5me1特异性抗体进行ChIP-seq分析，揭示了该修饰在转录延伸和反义RNA调控中的作用，表明其通过促进染色质松弛影响基因表达。
2. **文献名称**：*Monoubiquitination and methylation of histone H2B coordinate DNA damage response pathways*
**作者**：Johnson & Lee
**摘要**：通过H2BK5me1抗体验证，发现该修饰与H2B泛素化协同调控DNA损伤修复，表明其在招募修复蛋白(如BRCA1)中的关键角色。
3. **文献名称**：*Dynamic regulation of H2B monomethylation during cell cycle progression*
**作者**：Chen et al.
**摘要**：研究使用H2BK5me1特异性抗体进行免疫荧光和流式分析，证明该修饰水平在S期达到峰值，提示其与DNA复制和细胞周期检查点调控相关。
注：上述文献为虚拟示例，实际研究中建议通过PubMed或Google Scholar以关键词“H2BK5me1 antibody”或“Histone H2B K5 monomethylation”检索近期论文，并关注表观遗传学领域的高影响力期刊(如*Cell*、*Nature Structural & Molecular Biology*)。
The Histone H2B (Mono Methyl Lys5) antibody is a specialized tool used to detect monomethylation at lysine 5 (K5) of histone H2B, a post-translational modification (PTM) involved in epigenetic regulation. Histone H2B is one of the four core histones (H2A, H2B, H3. H4) that form the nucleosome structure, around which DNA is wrapped to compact chromatin. Methylation of histones, including H2B, plays a critical role in modulating chromatin accessibility and gene expression by recruiting effector proteins or altering nucleosome stability.
Monomethylation at H2B K5 (H2BK5me1) is a relatively less characterized mark compared to other histone modifications. Studies suggest it may be associated with transcriptional activation or repression, depending on the cellular context and interacting partners. For example, H2BK5me1 has been implicated in DNA repair processes and cell cycle regulation. This antibody is commonly used in techniques like chromatin immunoprecipitation (ChIP), immunoblotting, or immunofluorescence to map the spatial and temporal distribution of H2BK5me1. aiding in the study of its role in development, differentiation, or diseases such as cancer.
The specificity of the antibody is critical, as cross-reactivity with similar methylated residues (e.g., H2BK4me1 or H3K4me1) must be ruled out through validation assays. Researchers often use knockout cell lines or peptide competition experiments to confirm target specificity. Understanding H2BK5me1 dynamics contributes to broader insights into epigenetic mechanisms and their therapeutic targeting.
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