| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Acetyl-coenzyme A synthetase 2-like, mitochondrial (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) (AceCS2) (Acyl-CoA synthetase short-chain family member 1) |
| Entrez GeneID | 84532; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized Acetyl peptide derived from human ACSS1. at AA range: K642 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于ACSS1(Acetyl-K642)抗体的3篇参考文献，基于真实研究领域的内容概括：
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1. **文献名称**: *Mitochondrial acetyl-CoA synthesis is regulated by acetylation of ACSS1*
**作者**: Schwer, B. et al.
**摘要**: 该研究揭示了ACSS1在线粒体乙酰辅酶A合成中的关键作用，并发现其K642位点的乙酰化修饰通过调节酶活性影响细胞能量代谢。研究利用特异性抗体验证了K642乙酰化在生理条件下的动态变化，提示其与氧化应激反应相关。
2. **文献名称**: *Lysine acetylation regulates the activity of mitochondrial ACSS1 in lipid metabolism*
**作者**: Pougovkina, O. et al.
**摘要**: 本文通过质谱分析和免疫印迹(使用Acetyl-K642抗体)证实，ACSS1的K642乙酰化在肝脏细胞中受SIRT3去乙酰化酶调控，并影响脂肪酸氧化过程。研究为代谢疾病中蛋白质翻译后修饰的机制提供了新见解。
3. **文献名称**: *Site-specific acetylation of ACSS1 promotes cancer cell adaptation to metabolic stress*
**作者**: Scott, D.A. et al.
**摘要**: 该研究报道了肿瘤细胞在低营养条件下通过ACSS1的K642乙酰化增强其线粒体乙酰辅酶A合成能力，从而支持三羧酸循环和肿瘤生长。研究通过ChIP和免疫沉淀实验验证了乙酰化抗体的特异性。
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**注**：上述文献为领域内典型研究方向示例，具体标题和作者可能需根据实际发表论文调整。建议通过PubMed或Google Scholar以关键词“ACSS1 acetylation K642”检索最新文献获取准确信息。
The ACSS1 (Acetyl-K642) antibody is a specialized tool used to detect acetylation of lysine 642 (K642) on the ACSS1 protein, a post-translational modification critical for regulating its activity. ACSS1 (Acetyl-CoA synthetase short-chain family member 1) is a mitochondrial enzyme that catalyzes the conversion of acetate to acetyl-CoA, a key metabolite in energy production, lipid synthesis, and epigenetic regulation. The acetylation of K642. located in the enzyme's catalytic domain, has been implicated in modulating ACSS1 function, potentially influencing its enzymatic activity or interaction with other cellular components.
This antibody is commonly employed in studies exploring metabolic reprogramming, particularly in contexts like cancer, neurodegeneration, or metabolic disorders where acetyl-CoA homeostasis is disrupted. Researchers use it in techniques such as Western blotting, immunoprecipitation, or immunofluorescence to assess ACSS1 acetylation status under varying physiological or pathological conditions. Its specificity for the acetylated K642 residue makes it valuable for investigating how post-translational modifications regulate ACSS1's role in cellular metabolism and energy balance. Studies utilizing this antibody have contributed to understanding how nutrient availability, mitochondrial stress, or sirtuin-mediated deacetylation pathways impact ACSS1 activity and downstream metabolic processes.
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