| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CT |
| Entrez GeneID | 2547; |
| WB Predicted band size | 69kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Acetyl peptide from human protein at AA range: 317 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Ku-7 (Acetyl Lys317)抗体的3篇参考文献示例(注：具体文献可能需要根据实际研究补充或调整)：
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1. **"Site-Specific Acetylation of the Ku70 Protein Regulates DNA Repair and Apoptosis"**
- **作者**: Smith J. et al.
- **摘要**: 研究揭示了Ku70蛋白(包括Lys317位点)的乙酰化修饰在DNA损伤修复和细胞凋亡中的调控作用，并利用Ku-7 (Acetyl Lys317)抗体验证了该修饰在癌症细胞模型中的动态变化。
2. **"Development of a Novel Antibody Targeting Acetylated Ku70 for Epigenetic Studies"**
- **作者**: Lee H. et al.
- **摘要**: 报道了一种特异性识别Ku70蛋白Lys317位点乙酰化的抗体(Ku-7)的开发和验证，通过免疫印迹和免疫沉淀实验证明其在表观遗传调控研究中的应用潜力。
3. **"Acetylation-Dependent Regulation of Ku70 Subcellular Localization and Function"**
- **作者**: Garcia R. et al.
- **摘要**: 探讨了Ku70乙酰化(包括Lys317)对其核质转位和DNA修复功能的影响，使用Ku-7抗体证实了乙酰化修饰在氧化应激条件下的关键作用。
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**注意**：以上文献为示例性质，实际研究中可能需要通过PubMed、Google Scholar等平台检索具体论文。建议使用关键词 **"Ku70 acetylation Lys317"** 或 **"Ku-7 antibody acetyl Lys317"** 进一步查阅最新文献。
The Ku-7 (Acetyl Lys317) antibody is a specific immunological tool designed to detect the acetylated form of lysine 317 (K317) on the Ku70 protein, a critical subunit of the Ku heterodimer (Ku70/Ku80). This dimer plays a central role in DNA repair via the non-homologous end-joining (NHEJ) pathway, which addresses double-strand breaks (DSBs) in DNA. Post-translational modifications, such as acetylation, regulate Ku70's functions, including its interactions with repair proteins and chromatin. Acetylation at K317 has been implicated in modulating Ku70’s activity, potentially influencing DNA repair efficiency, apoptosis, and cellular responses to genotoxic stress.
The Ku-7 antibody is commonly used in research to study the dynamic regulation of Ku70 acetylation under conditions like ionizing radiation, chemotherapy, or histone deacetylase (HDAC) inhibitor treatments. By employing techniques such as Western blotting, immunoprecipitation, or immunofluorescence, researchers can assess acetylation-dependent changes in Ku70 localization, protein interactions, or repair complex assembly. This antibody has been particularly valuable in cancer biology, where dysregulated DNA repair mechanisms contribute to tumor progression or therapeutic resistance. Understanding K317 acetylation may reveal insights into cellular stress adaptation, genome stability, and strategies to sensitize cancer cells to DNA-damaging agents. Validation of the antibody typically includes testing in acetyltransferase-overexpressing or deacetylase-inhibited cell models to confirm specificity for the acetylated epitope.
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