| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLEC4F |
| Uniprot No | Q8N1N0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MDGEAVRFCTDNQCVSLHPQEVDSVAMAPAAPKIPRLVQATPAFMAVTLVFSLVTLFVVVQQQTRPVPKPVQAVILGDNITGHLPFEPNNHHHFGREAEMRELIQTFKGHMENSSAWVVEIQMLKCRVDNVNSQLQVLGDHLGNTNADIQMVKGVLKDATTLSLQTQMLRSSLEGTNAEIQRLKEDLEKADALTFQTLNFLKSSLENTSIELHVLSRGLENANSEIQMLNASLETANTQAQLANSSLKNANAEIYVLRGHLDSVNDLRTQNQVLRNSLEGANAEIQGLKENLQNTNALNSQTQAFIKSSFDNTSAEIQFLRGHLERAGDEIHVLKRDLKMVTAQTQKANGRLDQTDTQIQVFKSEMENVNTLNAQIQVLNGHMKNASREIQTLKQGMKNASALTSQTQMLDSNLQKASAEIQRLRGDLENTKALTMEIQQEQSRLKTLHVVITSQEQLQRTQSQLLQMVLQGWKFNGGSLYYFSSVKKSWHEAEQFCVSQGAHLASVASKEEQAFLVEFTSKVYYWIGLTDRGTEGSWRWTDGTPFNAAQNKAPGSKGSCPLRKYIIVNSGMGACSFIDTPPCPWILSN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLEC4F重组蛋白的3篇代表性文献及其摘要概述：
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1. **文献名称**：**"CLEC4F is a regulatory C-type lectin receptor for Kupffer cells in liver immunity"**
**作者**：Zhou, J., et al.
**摘要**：该研究报道了CLEC4F在肝脏库普弗细胞中的特异性表达，并通过重组蛋白技术证实其结合病原体相关糖脂(如脂磷壁酸)的能力，揭示了其在调控肝脏抗细菌免疫中的关键作用。
2. **文献名称**：**"Structural basis for recognition of sulfated glycosaminoglycans by CLEC4F"**
**作者**：Yamasaki, S., et al.
**摘要**：通过表达CLEC4F重组蛋白并进行X射线晶体学分析，阐明了其与硫酸化糖胺聚糖的相互作用机制，为CLEC4F在识别肝细胞损伤释放的内源性配体中的功能提供结构依据。
3. **文献名称**：**"Recombinant CLEC4F protein inhibits hepatitis B virus infection in vitro by targeting viral envelope glycoproteins"**
**作者**：Li, Q., et al.
**摘要**：研究利用哺乳动物系统表达CLEC4F重组蛋白，发现其通过直接结合乙肝病毒表面抗原(HBsAg)阻断病毒进入肝细胞，为开发抗病毒疗法提供了实验依据。
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**注**：以上文献信息为模拟创作，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
CLEC4F (C-type lectin domain family 4 member F) is a transmembrane protein belonging to the C-type lectin receptor (CLR) family, which plays critical roles in immune recognition and response. It is primarily expressed in liver-resident macrophages, known as Kupffer cells, and to a lesser extent in certain myeloid cells. Structurally, CLEC4F contains a conserved carbohydrate-recognition domain (CRD) that enables calcium-dependent binding to specific glycan structures, particularly those associated with pathogens or damaged cells. This receptor is implicated in pathogen sensing, immune modulation, and tissue homeostasis through interactions with endogenous or microbial ligands.
Recombinant CLEC4F protein refers to the engineered form of this lectin, typically produced in vitro using expression systems like mammalian cells or bacteria (e.g., E. coli with proper glycosylation considerations). Its production allows researchers to study ligand-receptor interactions, signaling pathways, and functional mechanisms in controlled settings. Studies have shown CLEC4F recognizes galactose/N-acetylgalactosamine (Gal/GalNAc) residues and may bind to lipoteichoic acid from Gram-positive bacteria, suggesting a role in antibacterial immunity. It has also been linked to clearance of apoptotic cells and modulation of liver inflammation.
Research applications include investigating liver diseases (e.g., fibrosis, hepatitis), host-pathogen interactions, and immune tolerance. Its recombinant form is valuable for developing diagnostic tools, therapeutic antibodies, or vaccine adjuvants by leveraging its target-binding specificity. Current challenges involve clarifying its precise physiological ligands and downstream signaling cascades, areas where recombinant protein studies provide essential insights.
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