| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/300 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | S6; RPT3; TBP7; TBP-7; MIP224 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PSMC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SRSF5抗体的参考文献示例(注：以下为虚构示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*"SRSF5 modulates alternative splicing in hepatocellular carcinoma progression"*
**作者**：Chen L. et al.
**摘要**：研究通过Western blot和免疫组化使用SRSF5抗体，揭示其在肝癌中异常表达并通过调控剪接因子促进肿瘤转移。
2. **文献名称**：*"A monoclonal antibody against SRSF5 reveals its dynamic localization in nuclear speckles"*
**作者**：Tanaka R. et al.
**摘要**：开发了一种高特异性小鼠抗SRSF5抗体，用于免疫荧光和流式细胞术，证实SRSF5在细胞核内的动态分布与RNA加工相关。
3. **文献名称**：*"SRSF5 regulates viral RNA splicing during influenza A infection"*
**作者**：Johnson M. et al.
**摘要**：利用SRSF5抗体进行免疫沉淀实验，发现其与流感病毒RNA结合并调控病毒基因剪接，影响病毒复制效率。
4. **文献名称**：*"CRISPR screening identifies SRSF5 as a mediator of chemotherapy resistance"*
**作者**：Wang Y. et al.
**摘要**：结合SRSF5抗体进行功能验证实验，发现其在卵巢癌细胞中通过剪接调控基因增强化疗耐药性。
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**提示**：实际研究中，建议通过PubMed或Google Scholar以“SRSF5 antibody”及“SRSF5 function”等关键词检索最新文献，并关注抗体应用(如WB/IHC/IP)及疾病模型相关研究。
**Background of SRSF5 Antibody**
SRSF5 (Serine/Arginine-Rich Splicing Factor 5), also known as SRP40 or HRS, is a member of the SR protein family, which plays critical roles in pre-mRNA splicing. These proteins are characterized by arginine/serine-rich (RS) domains and are essential for recognizing splice sites, regulating alternative splicing, and coupling splicing with other mRNA processing steps. SRSF5 specifically promotes exon inclusion by binding to exonic or intronic splicing enhancer elements, influencing transcript diversity and protein isoform expression.
Antibodies targeting SRSF5 are vital tools for studying its expression, localization, and function in RNA metabolism. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate SRSF5’s role in cellular processes, including cell cycle regulation, stress responses, and viral infection. Dysregulation of SRSF5 has been implicated in diseases such as cancer, where aberrant splicing contributes to tumor progression. Researchers utilize SRSF5 antibodies to explore its mechanistic contributions to splicing-related pathologies and potential therapeutic targets. These antibodies are also employed in model organisms to dissect developmental and tissue-specific splicing programs, underscoring their importance in both basic and translational research.
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