| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OPN2; Rhodopsin; Opsin-2 |
| Entrez GeneID | 6010; |
| WB Predicted band size | 39kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Rhodopsin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Rhodopsin抗体的参考文献(示例为模拟内容，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: *"Monoclonal antibodies against rhodopsin: characterization and cross-reactivity studies"*
**作者**: Molday RS, MacKenzie D
**摘要**: 该研究报道了针对牛视紫红质(Rhodopsin)的单克隆抗体制备，并通过免疫印迹和免疫组化验证其特异性。抗体能识别天然和变性的Rhodopsin，适用于视网膜组织的定位分析。
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2. **文献名称**: *"Anti-rhodopsin antibodies in retinal degeneration: a biomarker for disease progression"*
**作者**: Adamian M, et al.
**摘要**: 探讨了视网膜变性小鼠模型中抗Rhodopsin抗体的表达与疾病进展的关系，发现抗体水平与光感受器细胞凋亡呈正相关，提示其作为潜在生物标志物的价值。
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3. **文献名称**: *"Structural insights into rhodopsin-antibody interactions using cryo-EM"*
**作者**: Smith JJ, et al.
**摘要**: 通过冷冻电镜技术解析了抗Rhodopsin抗体与视紫红质蛋白的结合表位，揭示了抗体在稳定Rhodopsin构象中的作用，为光信号转导机制研究提供新视角。
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如需具体文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词："rhodopsin antibody", "anti-rhodopsin immunoassay", 并筛选近5-10年高被引论文。
Rhodopsin antibodies are essential tools in vision research, targeting the light-sensitive G protein-coupled receptor (GPCR) found in rod photoreceptor cells of the retina. Rhodopsin, comprising the protein opsin bound to the chromophore 11-cis-retinal, plays a central role in phototransduction, converting light into electrical signals. Mutations in the RHO gene encoding rhodopsin are linked to retinal degenerative diseases like retinitis pigmentosa (RP), driving interest in developing specific antibodies to study its expression, localization, and function.
The development of rhodopsin antibodies began in the late 20th century, leveraging recombinant protein technology and synthetic peptides to generate polyclonal or monoclonal variants. These antibodies are validated using knockout models or rhodopsin-deficient tissues to ensure specificity. Common applications include immunohistochemistry (IHC) for retinal tissue analysis, Western blotting to assess protein expression, and immunofluorescence for subcellular localization in photoreceptors.
In disease research, rhodopsin antibodies help characterize pathogenic mutations, evaluate experimental therapies (e.g., gene editing or pharmacological interventions), and monitor protein misfolding in RP models. Their utility extends to diagnostic assays and biomarker studies. However, challenges persist, including cross-reactivity with homologous GPCRs and variability due to post-translational modifications. Ongoing efforts focus on improving antibody specificity and standardization to enhance reproducibility in preclinical studies.
Overall, rhodopsin antibodies remain indispensable for unraveling retinal biology and advancing therapies for inherited blindness.
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