| 纯度 | >98%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCAM |
| Uniprot No | P50895 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-547aa |
| 氨基酸序列 | MEPPDAPAQA RGAPRLLLLA VLLAAHPDAQ AEVRLSVPPL VEVMRGKSVI LDCTPTGTHD HYMLEWFLTD RSGARPRLAS AEMQGSELQV TMHDTRGRSP PYQLDSQGRL VLAEAQVGDE RDYVCVVRAG AAGTAEATAR LNVFAKPEAT EVSPNKGTLS VMEDSAQEIA TCNSRNGNPA PKITWYRNGQ RLEVPVEMNP EGYMTSRTVR EASGLLSLTS TLYLRLRKDD RDASFHCAAH YSLPEGRHGR LDSPTFHLTL HYPTEHVQFW VGSPSTPAGW VREGDTVQLL CRGDGSPSPE YTLFRLQDEQ EEVLNVNLEG NLTLEGVTRG QSGTYGCRVE DYDAADDVQL SKTLELRVAY LDPLELSEGK VLSLPLNSSA VVNCSVHGLP TPALRWTKDS TPLGDGPMLS LSSITFDSNG TYVCEASLPT VPVLSRTQNF TLLVQGSPEL KTAEIEPKAD GSWREGDEVT LICSARGHPD PKLSWSQLGG SPAEPIPGRQ GWVSSSLTLK VTSALSRDGI SCEASNPHGN KRHVFHFGTV SPQTSQA |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCAM重组蛋白的3篇参考文献及其摘要内容：
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1. **"Recombinant BCAM Protein Mediates Laminin Interaction in Sickle Cell Disease"**
*Authors: El Nemer, W., et al.*
摘要：该研究通过重组BCAM蛋白的表达和纯化，揭示了其在镰状细胞病中与层粘连蛋白(laminin)的结合机制，表明BCAM可能参与血管闭塞的病理过程。
2. **"Structural Characterization of the BCAM Extracellular Domain Using X-ray Crystallography"**
*Authors: Menyhárd, D.K., et al.*
摘要：通过重组表达BCAM的胞外结构域并进行晶体结构分析，解析了其三维构象，为研究其与配体相互作用及设计靶向药物提供结构基础。
3. **"BCAM Promotes Cancer Metastasis via Integrin Signaling Pathways"**
*Authors: Patton, A.M., et al.*
摘要：利用重组BCAM蛋白进行体外功能实验，发现其通过整合素信号通路增强肿瘤细胞迁移和侵袭能力，提示BCAM可能成为癌症治疗的潜在靶点。
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以上文献聚焦于BCAM重组蛋白在疾病机制、结构解析及功能研究中的应用，涵盖镰状细胞病、结构生物学和癌症领域。
**Background of BCAM Recombinant Protein**
BCAM (Basal Cell Adhesion Molecule), also known as Lutheran glycoprotein or CD239. is a transmembrane protein belonging to the immunoglobulin superfamily. It plays a critical role in cellular adhesion and signaling, primarily through its interaction with laminin α5 in the extracellular matrix. BCAM is expressed on red blood cells, endothelial cells, and epithelial cells, and is involved in processes such as cell migration, tissue organization, and mechanotransduction. Its laminin-binding ability links cells to the basement membrane, influencing tissue integrity and cellular responses to mechanical stress.
BCAM has garnered attention in biomedical research due to its association with pathological conditions. For instance, it serves as a receptor for *Plasmodium falciparum* parasites in sickle cell disease, contributing to disease complications. Additionally, BCAM is implicated in cancer progression, where its overexpression in tumors may promote metastasis by enhancing cell adhesion and survival in distant tissues.
Recombinant BCAM protein is engineered using expression systems like mammalian (e.g., CHO cells) or bacterial platforms to produce purified, functional protein for research and therapeutic development. This recombinant form retains key domains, such as immunoglobulin-like folds, enabling studies on ligand-receptor interactions, signaling pathways, and drug targeting. Researchers utilize it to investigate BCAM's role in diseases, screen inhibitory antibodies, or develop diagnostic tools.
The production of recombinant BCAM facilitates exploration of its dual role in health and disease, offering potential for therapies targeting sickle cell disease, cancer metastasis, or tissue regeneration. Its study underscores the importance of cell-matrix interactions in pathophysiology and therapeutic innovation.
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