Rabbit Polyclonal FAM11A Antibody

+ +

•  

   
     Western blot analysis of LEKTI expression in HeLa whole cell lysates.    

•  

   
     Immunofluorescent analysis of FAM11A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.    

•  

   
     Immunohistochemical analysis of FAM11A staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.    

The FAM11A (Family with Sequence Similarity 11 Member A) protein, encoded by the FAM11A gene, is a poorly characterized protein with limited functional information available in current literature. It is ubiquitously expressed in human tissues, with higher levels observed in the testis, brain, and lymphoid organs. Structural analyses suggest it contains putative nuclear localization signals, hinting at potential roles in nuclear processes. Emerging studies associate FAM11A with cellular proliferation and cancer progression, particularly in breast and ovarian cancers, where its overexpression correlates with poor prognosis.

FAM11A antibodies are primarily utilized as research tools to investigate the protein's localization, expression patterns, and molecular interactions. Commercial antibodies are typically developed against specific epitopes, often targeting the N-terminal region (e.g., amino acids 1-150). These antibodies enable applications such as Western blotting, immunohistochemistry, and immunofluorescence. Validation remains challenging due to the lack of well-established biological functions and interacting partners. Recent proteomic studies suggest potential involvement in DNA damage response pathways, though mechanistic details require further exploration. As research progresses, FAM11A antibodies may gain importance in elucidating its role in cellular homeostasis and disease pathogenesis.