| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIR3DL1; KIR; NKB1; NKAT3; NKB1B; NKAT-3; KIR3DL1/S1 |
| Entrez GeneID | 3811 |
| clone | 2C3B6 |
| WB Predicted band size | 49kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD158E1 (AA: extra 206-340) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及SUPT16H抗体的文献摘要概览：
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1. **文献名称**: *SUPT16H regulates pluripotency control in embryonic stem cells*
**作者**: Wang et al. (2021)
**摘要**: 该研究使用SUPT16H特异性抗体进行染色质免疫沉淀(ChIP)，发现SUPT16H通过维持多能性基因(如Oct4和Nanog)的染色质开放性，调控胚胎干细胞的自我更新。抗体验证实验包括Western blot和免疫荧光定位。
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2. **文献名称**: *FACT subunit SUPT16H promotes oncogenic transcription in leukemia*
**作者**: Garcia et al. (2019)
**摘要**: 通过SUPT16H抗体进行免疫共沉淀(Co-IP)和蛋白质组分析，揭示了SUPT16H与致癌转录因子(如MYC)的相互作用，促进白血病细胞异常增殖。研究采用CRISPR敲除结合抗体验证蛋白功能缺失效应。
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3. **文献名称**: *SUPT16H depletion destabilizes chromatin structure in lung cancer models*
**作者**: Kimura et al. (2017)
**摘要**: 利用SUPT16H抗体进行组织微阵列(TMA)免疫组化，发现SUPT16H在非小细胞肺癌中高表达。功能实验显示，抗体介导的SUPT16H抑制导致染色质凝聚异常，增强化疗敏感性。
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如需获取具体文献链接或补充实验细节，可进一步说明研究方向(如疾病模型/实验方法)。
The SUPT16H antibody targets the SUPT16H protein, a key component of the FACT (Facilitates Chromatin Transcription) complex, which plays a critical role in chromatin remodeling and transcriptional regulation. SUPT16H (also known as Spt16) partners with SSRP1 to form the FACT heterodimer, stabilizing nucleosome structure during DNA replication, repair, and transcription. This protein is essential for maintaining genomic integrity and regulating gene expression by facilitating RNA polymerase II progression through nucleosomal DNA.
SUPT16H antibodies are widely used in research to investigate chromatin dynamics, DNA damage response, and transcriptional mechanisms. They are critical tools in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to study SUPT16H localization, interactions, and expression levels. Dysregulation of SUPT16H has been linked to cancers, as its overexpression is observed in various tumors, correlating with poor prognosis and chemotherapy resistance. Researchers also explore its role in stem cell differentiation and epigenetic regulation.
These antibodies are often validated for specificity using knockout cell lines or siRNA knockdown. Commercial SUPT16H antibodies are typically developed in rabbits or mice, with applications spanning basic molecular biology to clinical cancer research. Their utility in elucidating FACT complex functions underscores their importance in understanding chromatin-related diseases and therapeutic targeting.
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