Rabbit Polyclonal p22 Antibody

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     Western blot analysis of POLR3A expression in K562, MCF7, COS7 whole cell lysates.    

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     Immunofluorescent analysis of p220 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.    

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     Immunohistochemical analysis of p220 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.    

The p22 antibody targets the p22phox protein, a critical subunit of the NADPH oxidase complex, which plays a central role in generating reactive oxygen species (ROS). This 22-kDa protein, encoded by the *CYBA* gene, forms a heterodimer with gp91phox (NOX2) to create cytochrome b558. the membrane-bound core of the NADPH oxidase. Primarily expressed in phagocytes, this complex is essential for microbial killing via the "respiratory burst." Mutations in *CYBA* disrupt oxidase assembly, leading to chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by recurrent infections.

p22phox also interacts with other NOX isoforms (e.g., NOX1. NOX3. NOX4), linking it to ROS-mediated signaling in non-phagocytic cells, including endothelial, vascular smooth muscle, and fibroblasts. Dysregulated ROS production involving p22phox is implicated in cardiovascular diseases (e.g., hypertension, atherosclerosis), inflammation, and cancer.

Anti-p22 antibodies are vital tools in research and diagnostics. They enable detection of p22phox expression via Western blotting, immunofluorescence, or flow cytometry, aiding in CGD diagnosis by identifying protein deficiencies. Additionally, these antibodies help study ROS-related pathways in cellular models, clarifying p22phox's role in oxidative stress and disease mechanisms. Their specificity ensures accurate assessment of NADPH oxidase activity and subunit interactions, making them indispensable for both basic research and clinical applications.