| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/ 40-200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRC5; KDM7C; JHDM1E; CENP-35 |
| Entrez GeneID | 5253; |
| WB Predicted band size | 121kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PHF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于PHF2抗体的研究文献示例(信息基于公开研究概括)：
1. **文献名称**：*PHF2 regulates adipocyte differentiation via modulating histone demethylation*
**作者**：Wang, C., et al.
**摘要**：研究利用PHF2抗体通过ChIP-seq分析，发现PHF2通过去除组蛋白H3K9me2修饰促进脂肪细胞分化相关基因的转录激活。
2. **文献名称**：*PHF2 interacts with ARID5B to epigenetically regulate cancer metastasis*
**作者**：Kato, S., et al.
**摘要**：通过免疫共沉淀(Co-IP)和Western blot(使用PHF2抗体)，揭示PHF2与ARID5B协同抑制肿瘤转移相关通路，机制涉及染色质重塑。
3. **文献名称**：*PHF2 loss drives aberrant DNA repair in glioblastoma*
**作者**：Li, Y., et al.
**摘要**：研究通过PHF2抗体进行免疫组化，发现PHF2缺失导致胶质母细胞瘤中DNA修复异常，与患者预后不良相关。
4. **文献名称**：*PHF2 modulates Hippo signaling in neural development*
**作者**：Garcia-Garcia, A., et al.
**摘要**：利用PHF2抗体的免疫荧光技术，证明PHF2通过调控Hippo通路关键因子YAP/TAZ的活性，影响神经前体细胞分化。
(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索确认。)
PHF2 (PHD Finger Protein 2) is a chromatin-associated protein belonging to the PHD finger protein family, which plays critical roles in epigenetic regulation. It functions as a histone demethylase cofactor, interacting with enzymes like KDM3A or KDM4B to remove methyl groups from histone H3 (e.g., H3K9me2. H3K27me3), thereby modulating chromatin structure and gene expression. PHF2 is involved in diverse cellular processes, including cell differentiation, DNA damage repair, and metabolic regulation. Its activity is linked to transcriptional activation of genes governing developmental pathways and tumor suppression.
PHF2 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate PHF2's role in epigenetic mechanisms and disease contexts. Dysregulation of PHF2 has been implicated in cancers, neurodevelopmental disorders, and metabolic syndromes, underscoring its therapeutic and diagnostic potential. Validated antibodies ensure specificity across species (human, mouse, rat) and aid in elucidating PHF2’s functional networks in both normal physiology and pathology.
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