Rabbit Polyclonal GPR89A/GPR89B Antibody

GPR89A (Golgi pH Regulator 89A) and GPR89B are homologous transmembrane proteins belonging to the G protein-coupled receptor (GPCR) superfamily, though their exact signaling mechanisms remain unclear. Primarily localized to the Golgi apparatus, they regulate organelle pH homeostasis and glycosylation processes. GPR89A is ubiquitously expressed, while GPR89B shows tissue-specific expression, notably in the gastrointestinal tract. Antibodies targeting GPR89A/GPR89B are critical tools for studying their subcellular distribution, protein interactions, and functional roles in vesicular trafficking, secretory pathways, and autophagy. These antibodies are typically validated via knockout controls in techniques like immunofluorescence, Western blotting, or immunohistochemistry. Structural studies using these reagents suggest both proteins possess six transmembrane domains with luminal N-termini, distinguishing them from canonical GPCRs. Dysregulation of GPR89A/B has been implicated in cancers, neurodegenerative diseases, and metabolic disorders, driving interest in therapeutic targeting. However, antibody specificity challenges persist due to high sequence similarity (≈70%) between the paralogs. Recent efforts focus on developing isoform-specific antibodies to dissect their non-redundant functions. Commercial antibodies often target divergent regions like the C-terminal cytoplasmic tails. Proper validation remains essential, as nonspecific binding to Golgi-resident proteins is a common concern.