| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DECTIN1 |
| Uniprot No | Q9BXN2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247aa |
| 氨基酸序列 | MEYHPDLENLDEDGYTQLHFDSQSNTRIAVVSEKGSCAASPPWRLIAVILGILCLVILVIAVVLGTMAIWRSNSGSNTLENGYFLSRNKENHSQPTQSSLEDSVTPTKAVKTTGVLSSPCPPNWIIYEKSCYLFSMSLNSWDGSKRQCWQLGSNLLKIDSSNELGFIVKQVSSQPDNSFWIGLSRPQTEVPWLWEDGSTFSSNLFQIRTTATQENPSPNCVWIHVSVIYDQLCSVPSYSICEKKFSM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DECTIN1(Dectin-1)重组蛋白的3篇参考文献，按研究主题分类整理：
---
1. **文献名称**：*Dectin-1 is a major β-glucan receptor on macrophages*
**作者**：Brown GD, et al.
**摘要**：首次报道Dectin-1作为巨噬细胞表面β-葡聚糖的关键受体，研究通过重组蛋白表达证实其与真菌细胞壁成分的结合能力，揭示了其在先天免疫中的核心作用。
2. **文献名称**：*Structural characterization of recombinant Dectin-1 extracellular domain and its interaction with β-glucans*
**作者**：Adachi Y, et al.
**摘要**：利用重组Dectin-1胞外域蛋白进行X射线晶体学分析，解析了其与β-葡聚糖结合的分子机制，为开发靶向免疫调节药物提供结构基础。
3. **文献名称**：*Recombinant Dectin-1 modulates cytokine production in dendritic cells via Syk kinase signaling*
**作者**：LeibundGut-Landmann S, et al.
**摘要**：通过重组Dectin-1蛋白刺激树突状细胞，证明其通过激活Syk激酶通路诱导IL-10和IL-23分泌，阐明了其在抗真菌与自身免疫疾病中的双重调控作用。
---
**提示**：如需具体实验方案或应用案例，可进一步检索关于重组Dectin-1在疫苗佐剂开发或肿瘤免疫治疗中的研究(如结合CAR-T技术的文献)。
Dectin-1 (also known as CLEC7A) is a C-type lectin receptor predominantly expressed on immune cells, including dendritic cells, macrophages, and neutrophils. It plays a critical role in recognizing β-glucans, carbohydrate components found in fungal cell walls, thereby serving as a key pattern recognition receptor in antifungal immunity. Structurally, Dectin-1 contains an extracellular carbohydrate-recognition domain (CRD), a transmembrane region, and a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM)-like signaling domain. Upon ligand binding, Dectin-1 triggers intracellular signaling cascades, often involving Syk kinase, to induce phagocytosis, reactive oxygen species production, and cytokine secretion, which collectively orchestrate innate and adaptive immune responses against fungal pathogens.
Recombinant Dectin-1 proteins are engineered versions of this receptor, typically produced in vitro using mammalian or insect cell expression systems. These proteins often include the extracellular CRD region to preserve ligand-binding functionality, sometimes fused with tags (e.g., Fc or His-tags) for purification or detection. Researchers utilize recombinant Dectin-1 to study receptor-ligand interactions, immune signaling mechanisms, and host-pathogen dynamics. It has also been explored for therapeutic applications, such as enhancing antifungal immunity or modulating inflammatory diseases. Additionally, recombinant Dectin-1 serves as a tool in vaccine development, where β-glucan recognition can be leveraged to boost adjuvant activity. Its role in autoimmune conditions and cancer immunotherapy is under investigation, reflecting its broad relevance in immunology and translational medicine.
×
