| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Fatty acid 2-hydroxylase (EC 1.-.-.-) (Fatty acid alpha-hydroxylase) |
| Entrez GeneID | 79152; |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human FA2H. at AA range: 101-150 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于FA2H抗体的3篇参考文献，基于公开数据整理：
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1. **文献名称**: *Mutations in FA2H underlie a novel form of neurodegeneration with brain iron accumulation (NBIA)*
**作者**: Kruer MC, et al.
**摘要**: 该研究首次将FA2H基因突变与神经退行性疾病(伴有脑铁沉积)联系起来，利用FA2H抗体检测患者脑组织中FA2H蛋白表达缺失，揭示其在髓鞘脂质代谢中的关键作用。
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2. **文献名称**: *Fatty acid 2-hydroxylation in mammalian sphingolipid metabolism*
**作者**: Hama H.
**摘要**: 文章系统综述FA2H酶在鞘脂合成中的功能，提及开发FA2H特异性抗体的方法，并用于小鼠模型脑组织分析，证明其在少突胶质细胞中的高表达。
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3. **文献名称**: *FA2H-dependent fatty acid 2-hydroxylation in postnatal adrenal development*
**作者**: Zoller I, et al.
**摘要**: 通过FA2H抗体免疫组化技术，研究发现FA2H在小鼠肾上腺皮质细胞中活跃表达，调控特定羟化脂肪酸生成，影响细胞膜结构与激素分泌功能。
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**备注**：部分文献可能侧重FA2H基因功能而非抗体本身，建议通过PubMed或Google Scholar以“FA2H antibody” + “application”/“characterization”等关键词进一步筛选实验方法学相关研究。
The fatty acid 2-hydroxylase (FA2H) antibody is a tool used to detect FA2H, an enzyme critical for synthesizing 2-hydroxy fatty acids in sphingolipids, particularly in myelin sheaths. FA2H catalyzes the hydroxylation of free fatty acids, generating 2-hydroxy species that are incorporated into galactocerebrosides and sulfatides—key components of neuronal and glial cell membranes. These lipids contribute to membrane stability, myelin integrity, and cellular signaling. Dysregulation of FA2H is linked to neurodegenerative disorders like hereditary spastic paraplegia type 35 (SPG35) and leukodystrophies.
FA2H antibodies are widely used in research to study the enzyme's expression, localization, and function in tissues, notably the brain and spinal cord. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess FA2H levels in disease models or genetic mutants. Some antibodies target specific epitopes, such as the C-terminal domain or catalytic regions, aiding in distinguishing functional isoforms or mutations. Recent studies also explore FA2H's role in lipid metabolism and its potential as a biomarker for neurological conditions. Commercial FA2H antibodies are typically validated in human, mouse, or rat samples, supporting both basic research and preclinical investigations into myelin-related pathologies.
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