Rabbit Polyclonal TCF7L1 Antibody

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     Gel: 8%SDS-PAGE  Lysate: 40 μg, Lane: Hepg2 cell lysate, Primary antibody:TCF7L1 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

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     The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TCF7L1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)    

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     The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using TCF7L1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)    

**Background of TCF7L1 Antibody**

TCF7L1 (T-cell factor 7-like 1), also known as TCF3. is a member of the TCF/LEF family of transcription factors that play critical roles in the Wnt/β-catenin signaling pathway. This pathway regulates key biological processes, including embryonic development, cell differentiation, and stem cell maintenance. TCF7L1 contains a high-mobility group (HMG) DNA-binding domain and a β-catenin interaction domain. Unlike other TCF members (e.g., TCF7L2), TCF7L1 primarily acts as a transcriptional repressor in the absence of Wnt signaling by recruiting co-repressors (e.g., Groucho/TLE) to silence target genes. Upon Wnt activation, β-catenin translocates to the nucleus, displaces co-repressors, and converts TCF7L1 into a transcriptional activator, driving expression of Wnt-responsive genes.

Antibodies targeting TCF7L1 are essential tools for studying its expression, localization, and function. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies help elucidate TCF7L1's role in development and diseases, particularly cancers (e.g., colorectal, breast) where dysregulated Wnt signaling is common. Monoclonal and polyclonal TCF7L1 antibodies are available, often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers must verify antibody performance in their experimental systems due to potential cross-reactivity with other TCF family members. Reliable TCF7L1 antibodies remain crucial for dissecting Wnt pathway dynamics and therapeutic targeting.