关键信息
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基因名
PCDH19
- 应用
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别名
PCDH19；KIAA1313；Protocadherin-19
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8TAB3
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表达区间
22-678aa
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氨基酸序列
LINLKYSVEEEQRAGTVIANVAKDAREAGFALDPRQASAFRVVSNSAPHLVDINPSSGLLVTKQKIDRDLLCRQSPKCIISLEVMSSSMEICVIKVEIKDLNDNAPSFPAAQIELEISEAASPGTRIPLDSAYDPDSGSFGVQTYELTPNELFGLEIKTRGDGSRFAELVVEKSLDRETQSHYSFRITALDGGDPPRLGTVGLSIKVTDSNDNNPVFSESTYAVSVPENSPPNTPVIRLNASDPDEGTNGQVVYSFYGYVNDRTRELFQIDPHSGLVTVTGALDYEEGHVYELDVQAKDLGPNSIPAHCKVTVSVLDTNDNPPVINLLSVNSELVEVSESAPPGYVIALVRVSDRDSGLNGRVQCRLLGNVPFRLQEYESFSTILVDGRLDREQHDQYNLTIQARDGGVPMLQSAKSFTVLITDENDNHPHFSKPYYQVIVQENNTPGAYLLSVSARDPDLGLNGSVSYQIVPSQVRDMPVFTYVSINPNSGDIYALRSFNHEQTKAFEFKVLAKDGGLPSLQSNATVRVIILDVNDNTPVITAPPLINGTAEVYIPRNSGIGYLVTVVKAEDYDEGENGRVTYDMTEGDRGFFEIDQVNGEVRTTRTFGESSKSSYELIVVAHDHGKTSLSASALVLIYLSPALDAQESMGSVNLS
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分子量
100.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PCDH19 (Protocadherin 19) is a member of the cadherin superfamily and plays a critical role in regulating cell-cell interactions within the developing nervous system. Mutations in the PCDH19 gene have been implicated in a rare X-linked neurodevelopmental disorder known as PCDH19-related epilepsy, characterized by early-onset seizures and cognitive impairment, primarily affecting females due to X-inactivation patterns. Research into PCDH19 has gained momentum as understanding its structure and function could provide insights into underlying pathogenic mechanisms and potential therapeutic targets. The production of recombinant PCDH19 protein is essential for investigating its molecular characteristics, including binding affinities, structural properties, and interaction with other proteins. This study aims to generate and characterize PCDH19 recombinant protein, utilizing various expression systems to ensure proper folding and functionality. By examining its role in neuronal development and synaptic plasticity, this research contributes to a broader understanding of PCDH19's involvement in epilepsy and neurodevelopmental disorders, paving the way for future therapeutic strategies. Additionally, insights gained from PCDH19 protein interactions may benefit the fields of neurobiology and genetics, offering potential advancements in precision medicine approaches for patients affected by PCDH19-related disorders. Through this work, we hope to elucidate the multifaceted roles of PCDH19 in neuronal function and pathology, providing a foundational step toward the development of targeted interventions for affected individuals.
