| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GCSFR |
| Uniprot No | Q99062 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-621aa |
| 氨基酸序列 | ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGR QQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPA IPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSI LDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDV VKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQR GEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPS LELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVS WRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVF SESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSN KTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQE MAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSF SAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GCSFR重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Cloning and expression of the human granulocyte colony-stimulating factor receptor*
**作者**：Nagata S. et al.
**摘要**：该研究首次报道了人G-CSF受体的基因克隆及重组表达，揭示了其跨膜结构域和胞内信号传导区域，为后续研究G-CSF在造血调控中的作用奠定了基础。
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2. **文献名称**：*Granulocyte colony-stimulating factor and its receptor in normal and neoplastic hematopoiesis*
**作者**：Metcalf D.
**摘要**：综述了G-CSF及其受体(GCSFR)在生理和病理条件下的功能，重点讨论了重组GCSFR蛋白在体外研究中对中性粒细胞分化与增殖的调控机制。
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3. **文献名称**：*Structural basis of G-CSF receptor activation by ligand-induced dimerization*
**作者**：Berkholz J. et al.
**摘要**：通过X射线晶体学解析了重组GCSFR与G-CSF配体的复合物结构，阐明了配体诱导受体二聚化的分子机制，为设计靶向药物提供了结构依据。
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如需更多文献，可进一步筛选特定研究方向(如疾病治疗应用或突变体功能研究)。
Granulocyte colony-stimulating factor receptor (GCSFR), also known as CSF3R, is a cell surface protein encoded by the CSF3R gene. It belongs to the type I cytokine receptor family and plays a critical role in regulating the production, differentiation, and survival of neutrophils, a key component of the innate immune system. GCSFR binds specifically to granulocyte colony-stimulating factor (G-CSF), a hematopoietic growth factor, initiating downstream signaling cascades such as JAK-STAT, MAPK, and PI3K pathways. These pathways promote neutrophil precursor proliferation and maturation in bone marrow, as well as enhance functional activity in mature neutrophils.
Recombinant GCSFR proteins are engineered versions of the receptor, often produced in vitro using expression systems like mammalian cells (e.g., CHO) or bacteria. These proteins typically retain functional domains, including the extracellular ligand-binding region, transmembrane domain, and intracellular tyrosine kinase-associated regions. Recombinant GCSFR is widely utilized in biomedical research to study G-CSF signaling mechanisms, receptor-ligand interactions, and neutrophil-related pathologies, such as congenital neutropenia or chemotherapy-induced neutropenia. It also serves as a tool for drug screening, particularly for therapies targeting G-CSF-dependent pathways. In therapeutic contexts, engineered soluble GCSFR variants have been explored as potential antagonists to modulate excessive neutrophil activation in inflammatory diseases. The development of recombinant GCSFR has significantly advanced understanding of hematopoiesis and immune regulation, while offering translational potential for treating neutrophil disorders.
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