| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDLR |
| Uniprot No | P01130 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-788aa |
| 氨基酸序列 | AVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVR |
| 预测分子量 | 89.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于LDLR重组蛋白的参考文献示例(注：文献信息为示例性概括，部分内容可能存在虚构)：
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1. **"Crystal structure of the LDL receptor extracellular domain at acidic pH"**
*Authors: Springer TA et al.*
摘要：解析了低密度脂蛋白受体(LDLR)胞外结构域在酸性条件下的晶体结构，揭示了其与配体结合及释放的构象变化机制，为理解LDLR介导的胆固醇内吞提供结构基础。
2. **"Recombinant LDL receptor ligand-binding domain: Functional characterization and therapeutic potential"**
*Authors: Jeon H, Blacklow SC*
摘要：通过重组表达LDLR配体结合结构域，验证其与ApoB-100的结合活性，并探讨其在调节血浆LDL水平及治疗动脉粥样硬化中的潜在应用。
3. **"Expression and purification of functional human LDL receptor in mammalian cells"**
*Authors: Huang X et al.*
摘要：开发了基于哺乳动物细胞体系的重组人LDLR高效表达与纯化方法，证明纯化蛋白具备天然受体功能，可用于体外药物筛选及受体功能缺陷相关疾病研究。
4. **"Gene therapy for familial hypercholesterolemia using recombinant LDLR adenovirus"**
*Authors: Wang Y et al.*
摘要：利用携带重组LDLR基因的腺病毒载体治疗LDLR缺陷小鼠模型，显著降低血清胆固醇水平，为家族性高胆固醇血症的基因治疗提供实验依据。
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提示：实际文献需通过PubMed或Web of Science按关键词“recombinant LDL receptor”检索，并筛选高影响力期刊论文(如*Nature, Cell, JBC*等)。
The low-density lipoprotein receptor (LDLR) is a cell-surface glycoprotein critical for regulating cholesterol homeostasis by mediating the uptake of LDL particles, thereby reducing circulating LDL cholesterol levels. Dysfunctional LDLR activity is linked to hypercholesterolemia and cardiovascular diseases, notably familial hypercholesterolemia (FH), a genetic disorder characterized by impaired LDL clearance. To study and address these pathologies, recombinant LDLR proteins are produced using genetic engineering techniques. These proteins are generated by cloning the LDLR gene into expression vectors, which are then transfected into host systems like bacteria, yeast, or mammalian cells (e.g., CHO or HEK293). Post-translationally modified mammalian systems are preferred for producing functional LDLR with proper folding and ligand-binding activity.
Recombinant LDLR serves as a vital tool for investigating cholesterol metabolism mechanisms, receptor trafficking, and mutations causing FH. It is also explored in therapeutic strategies, including gene therapy and protein replacement, to restore LDLR function in FH patients. Additionally, it aids in drug screening for lipid-lowering therapies and serves as a reference in diagnostic assays. The choice of expression system balances cost, scalability, and biological relevance, with mammalian-derived LDLR retaining native-like properties despite higher production complexity. Overall, recombinant LDLR bridges basic research and clinical applications, offering insights into cardiovascular biology and potential treatments for cholesterol-related disorders.
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