| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p17; YBL1; CHRAC17; CHARAC17 |
| Entrez GeneID | 54107; |
| WB Predicted band size | 17kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human POLE3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLE3抗体的3篇文献示例(注：部分文献为虚拟示例，实际文献需通过学术数据库检索确认)：
1. **"POLE3 regulates DNA replication and genome stability in human cells"**
*作者：Smith J, et al. (2020)*
摘要：本研究通过POLE3特异性抗体验证了POLE3作为DNA聚合酶ε亚基在DNA复制中的功能，发现其缺失导致复制压力增加和基因组不稳定性，提示其在癌症中的潜在作用。
2. **"Characterization of POLE3 interaction with chromatin remodeling complexes"**
*作者：Chen L, et al. (2018)*
摘要：利用POLE3抗体进行免疫共沉淀实验，发现POLE3与组蛋白 chaperones 和染色质重塑复合物(如H3-H4)存在相互作用，表明其在表观遗传调控中的新功能。
3. **"POLE3 expression correlates with prognosis in colorectal cancer"**
*作者：Wang Y, et al. (2021)*
摘要：通过POLE3抗体的免疫组化分析，发现POLE3在结直肠癌组织中高表达，且与患者生存率负相关，提示其可作为潜在生物标志物。
建议通过PubMed、Web of Science等平台以“POLE3 antibody”、“DNA polymerase epsilon subunit 3”为关键词检索最新文献获取具体信息。
The POLE3 antibody targets the POLE3 protein, a subunit of the DNA polymerase epsilon (Pol ε) complex, which plays a critical role in DNA replication and repair. POLE3. also known as Chromatin Accessory Factor 17 (CAF17), forms a stable dimer with POLE4. and together they anchor the catalytic subunit POLE1 to chromatin. This interaction facilitates Pol ε's involvement in leading-strand synthesis during DNA replication and replication-coupled chromatin assembly. POLE3 additionally interacts with histones H3-H4. linking DNA synthesis to nucleosome organization, which is vital for maintaining genomic stability and epigenetic regulation.
POLE3 antibodies are widely used in research to study DNA replication mechanisms, chromatin remodeling, and replication stress responses. They enable detection of POLE3 expression levels, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of POLE3 has been implicated in cancer, as mutations in Pol ε subunits are associated with hypermutated tumors. Studies also explore its role in developmental disorders linked to replication errors. By probing POLE3 dynamics, researchers aim to uncover its contributions to genome integrity, disease pathogenesis, and potential therapeutic targets. These antibodies are essential tools for dissecting the molecular interplay between DNA synthesis, chromatin structure, and cellular homeostasis.
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