| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPEP1 |
| Uniprot No | P16444 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385aa |
| 氨基酸序列 | MWSGWWLWPL VAVCTADFFR DEAERIMRDS PVIDGHNDLP WQLLDMFNNR LQDERANLTT LAGTHTNIPK LRAGFVGGQF WSVYTPCDTQ NKDAVRRTLE QMDVVHRMCR MYPETFLYVT SSAGIRQAFR EGKVASLIGV EGGHSIDSSL GVLRALYQLG MRYLTLTHSC NTPWADNWLV DTGDSEPQSQ GLSPFGQRVV KELNRLGVLI DLAHVSVATM KATLQLSRAP VIFSHSSAYS VCASRRNVPD DVLRLVKQTD SLVMVNFYNN YISCTNKANL SQVADHLDHI KEVAGARAVG FGGDFDGVPR VPEGLEDVSK YPDLIAELLR RNWTEAEVKG ALADNLLRVF EAVEQASNLT QAPEEEPIPL DQLGGSCRTH YGYSS |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPEP1重组蛋白的3篇示例参考文献(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
1. **标题**: "Recombinant Human Dipeptidase-1 (DPEP1): Purification and Enzymatic Characterization"
**作者**: Smith A, et al.
**摘要**: 本研究报道了人源DPEP1重组蛋白在大肠杆菌中的高效表达与纯化方法，并分析了其水解特定二肽底物的酶动力学参数，证实重组蛋白具有与天然DPEP1相似的生物活性。
2. **标题**: "DPEP1 as a Novel Biomarker in Renal Fibrosis: Insights from Recombinant Protein Studies"
**作者**: Chen L, et al.
**摘要**: 通过体外表达DPEP1重组蛋白，研究发现其通过调节TGF-β信号通路参与肾小管间质纤维化进程，提示DPEP1可能成为慢性肾病治疗的潜在靶点。
3. **标题**: "Structural Analysis of DPEP1 Using X-ray Crystallography: Implications for Substrate Specificity"
**作者**: Yamamoto K, et al.
**摘要**: 首次解析了重组DPEP1蛋白的晶体结构(分辨率2.1Å)，揭示了其金属离子结合域和底物识别口袋的三维构象，为设计特异性抑制剂提供了结构基础。
建议通过PubMed或Web of Science以"DPEP1 recombinant protein"为关键词检索最新文献，重点关注其在代谢疾病、癌症转移或药物代谢领域的研究进展。
**Background of DPEP1 Recombinant Protein**
Dipeptidase 1 (DPEP1), also known as renal dipeptidase, is a membrane-bound glycoprotein belonging to the M19 metalloprotease family. It catalyzes the hydrolysis of specific dipeptides, including leukotriene D4 (LTD4) to LTE4. and plays a role in renal physiology, epithelial function, and inflammatory responses. DPEP1 is predominantly expressed in the kidney, liver, and lung tissues, with its enzymatic activity dependent on zinc ions. Its involvement in metabolic pathways, particularly in modulating bioactive lipid mediators like leukotrienes, links it to inflammatory diseases, fibrosis, and cancer progression.
Recombinant DPEP1 protein is engineered using genetic cloning techniques, typically expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. This protein retains the enzymatic activity of native DPEP1. enabling its use in functional studies, drug discovery, and diagnostic applications. Researchers employ DPEP1 recombinant protein to investigate its role in diseases such as acute kidney injury, chronic fibrosis, and malignancies, where dysregulated DPEP1 expression correlates with pathological outcomes.
Additionally, DPEP1 has garnered attention as a potential therapeutic target. Inhibitors or modulators of DPEP1 activity are being explored for treating inflammatory disorders or enhancing chemotherapeutic efficacy, as DPEP1 can metabolize certain prodrugs. The recombinant form also aids in antibody development for biomarker studies, given its association with disease-specific epitopes. Overall, DPEP1 recombinant protein serves as a critical tool for unraveling the protein's biological significance and translational potential in human health and disease.
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