| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PA2G4 |
| Uniprot No | Q9UQ80 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-394aa |
| 氨基酸序列 | SGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL KEGDLVKIDL GVHVDGFIAN VAHTFVVDVA QGTQVTGRKA DVIKAAHLCA EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ DAELKALLQS SASRKTQKKK KKKASKTAEN ATSGETLEEN EAGD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PA2G4重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Recombinant human PA2G4 (EBP1) inhibits cell proliferation and suppresses tumor growth in vivo"*
**作者**：Zhang Y, et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化重组人PA2G4蛋白，发现其能显著抑制多种癌细胞系的增殖，并通过动物模型证实其体内抗肿瘤活性，提示PA2G4可能通过调控细胞周期相关蛋白发挥作用。
2. **文献名称**：*"Structural and functional characterization of PA2G4 (EBP1) as a ribosome-associated protein in mRNA translation"*
**作者**：Lesser C, et al.
**摘要**：研究利用重组PA2G4蛋白进行结构解析和功能实验，发现其通过与核糖体亚基结合参与mRNA翻译调控，并揭示其C端结构域对蛋白-核糖体互作的关键作用。
3. **文献名称**：*"PA2G4 interacts with EGFR and regulates its downstream signaling in breast cancer cells"*
**作者**：Wang L, et al.
**摘要**：通过表达重组PA2G4蛋白并结合免疫共沉淀实验，证实PA2G4与表皮生长因子受体(EGFR)直接互作，抑制EGFR信号通路活化，从而影响乳腺癌细胞的迁移和侵袭能力。
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以上文献涵盖了PA2G4重组蛋白的功能、结构及在癌症中的机制研究，可作为相关领域的基础参考。如需更多文献或具体发表年份，可进一步补充关键词检索。
PA2G4 (Proliferation-Associated 2G4), also known as Ebp1. is a multifunctional protein encoded by the PA2G4 gene. It belongs to the DNAJB4/HSP40 family of heat shock proteins, though it lacks the classic J-domain typical of this family. PA2G4 plays diverse roles in cellular processes, including cell proliferation, differentiation, apoptosis, and transcriptional regulation. It exists in two major isoforms (p48 and p42) generated through alternative splicing, with p48 predominantly localized in the nucleus and p42 in the cytoplasm. PA2G4 interacts with key signaling molecules such as EGFR, HER2. and the retinoblastoma protein (Rb), modulating pathways like PI3K/AKT and MAPK. Its dual role as either an oncogene or tumor suppressor, depending on cellular context and post-translational modifications, has been highlighted in cancers like breast, prostate, and glioblastoma.
Recombinant PA2G4 is produced using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and therapeutic potential. Purified recombinant PA2G4 retains functional domains, including nuclear localization signals, RNA-binding motifs, and protein interaction sites, enabling research into its role in RNA metabolism, ribosome biogenesis, and stress response. Its involvement in cancer progression and chemotherapy resistance has spurred interest in targeting PA2G4 for diagnostics or treatments. Additionally, recombinant PA2G4 serves as an antigen for antibody development and a tool for elucidating mechanisms in neurodegenerative and metabolic disorders. Ongoing studies aim to clarify its context-dependent functions and therapeutic applications.
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