| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | CT1.1; MAGE1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MAGEA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Tubulin α抗体的3篇参考文献，按研究内容与时间顺序列举：
1. **Kilmartin, J.V., et al. (1982)**
*"Monoclonal antibodies to tubulin as probes for the detection of microtubule antigens in human cell lines."*
**摘要**：该研究首次报道了针对α-和β-tubulin的单克隆抗体的制备，并通过免疫荧光和免疫印迹验证其在检测人源细胞系微管结构中的应用，为后续微管研究提供了关键工具。
2. **Breitling, F., & Little, M. (1986)**
*"DM1A, a widely used monoclonal antibody for α-tubulin: characterization and immunolocalization."*
**摘要**：详细描述了单克隆抗体DM1A的制备及其对α-tubulin的特异性验证，证明其在免疫印迹(Western blot)和免疫细胞化学中的可靠性，成为后续研究中的标准内参抗体。
3. **Gadadhar, S., et al. (2017)**
*"Validation of antibodies for the specific detection of post-translationally modified α-tubulins."*
**摘要**：系统评估了多种α-tubulin抗体对乙酰化、去酪氨酸化等修饰形式的特异性，强调在研究中需根据实验目标(如细胞定位或翻译后修饰分析)选择合适的抗体。
4. **Joshi, S., et al. (2019)**
*"Antibody validation for Western blot: Importance of sample preparation and controls."*
**摘要**：以α-tubulin抗体为例，探讨了Western blot中抗体验证的关键因素，包括样品制备、对照设置及批次间差异的优化方法，为实验标准化提供指导。
这些文献涵盖了α-tubulin抗体的开发、特异性验证及在不同实验场景(如Western blot、免疫荧光)中的应用，兼顾经典研究与现代方法学建议。
Tubulin α antibodies are essential tools in cell biology research, targeting the α-subunit of tubulin, a key component of microtubules. Microtubules, composed of α- and β-tubulin heterodimers, form dynamic cytoskeletal structures critical for maintaining cell shape, intracellular transport, mitosis, and cell motility. The α-tubulin subunit binds GTP and interacts with β-tubulin to assemble into protofilaments, which further organize into hollow microtubule polymers.
These antibodies are widely used to study microtubule dynamics, localization, and expression across cell types and experimental conditions. Common applications include Western blotting, immunofluorescence microscopy, immunohistochemistry, and flow cytometry. Specific α-tubulin clones (e.g., DM1A, B-512) are validated for species cross-reactivity, including human, mouse, rat, and other model organisms. As α-tubulin exhibits constitutive expression in most cells, its antibodies often serve as loading controls in protein quantification assays, though variations due to post-translational modifications (e.g., acetylation, detyrosination) or disease states require careful interpretation.
Researchers also employ α-tubulin antibodies to investigate microtubule-related pathologies, such as neurodegenerative disorders and cancer, where microtubule stability influences drug sensitivity (e.g., taxanes, vinca alkaloids). Both monoclonal and polyclonal variants exist, offering flexibility in experimental design based on specificity and detection requirements.
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