| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC3A2 |
| Uniprot No | P08195 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 206-630aa |
| 氨基酸序列 | RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLK VKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVI LDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKD ASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYL SDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQL MLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSA NMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIR HWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREE GSPLELERLKLEPHEGLLLRFPYAAVDHHHHHH |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant SLC3A2 protein production and its role in amino acid transport"**
- 作者：Zhang et al. (2018)
- 摘要：研究通过大肠杆菌系统成功表达并纯化SLC3A2重组蛋白，验证其与轻链蛋白(如SLC7A5)的相互作用，并证明其在细胞膜上介导的胱氨酸和谷氨酰胺转运活性，为靶向氨基酸代谢的癌症治疗提供依据。
2. **"Structural and functional analysis of CD98hc (SLC3A2) in integrin signaling"**
- 作者：Fenczik et al. (2001)
- 摘要：利用重组SLC3A2蛋白探究其胞外结构域与整合素β1亚基的结合机制，发现其通过调控整合素信号通路影响细胞黏附和迁移，提示SLC3A2在肿瘤转移中的潜在作用。
3. **"SLC3A2 recombinant protein as a therapeutic target in glutamine-dependent cancers"**
- 作者：Cantor et al. (2017)
- 摘要：通过体外实验证实，阻断重组SLC3A2蛋白功能可抑制癌细胞对谷氨酰胺的摄取，导致代谢应激和凋亡，为开发针对SLC3A2的抑制剂提供理论支持。
4. **"Biochemical characterization of SLC3A2 glycosylation and its impact on protein stability"**
- 作者：Prasad et al. (2020)
- 摘要：研究真核表达系统中SLC3A2重组蛋白的糖基化修饰模式，发现N-糖基化对其细胞膜定位及与轻链蛋白的稳定性至关重要，为优化重组蛋白生产提供参考。
SLC3A2 recombinant protein is derived from the SLC3A2 gene, which encodes a transmembrane glycoprotein known as CD98 heavy chain (CD98hc). This protein plays a critical role in amino acid transport and cellular integrin signaling. SLC3A2 forms a heterodimeric complex with light chains of the SLC7 family (e.g., SLC7A5/LAT1 or SLC7A11/xCT), creating functional transporters that mediate the uptake of neutral or cationic amino acids. These transporters are essential for nutrient sensing, cellular proliferation, and redox balance, linking metabolic activity to processes like mTORC1 signaling.
Recombinant SLC3A2 is produced using expression systems (e.g., mammalian, insect, or bacterial cells) to generate purified protein for research. Its production enables studies on structure-function relationships, substrate specificity, and interactions with binding partners. Researchers use it to investigate amino acid transport mechanisms in physiological and pathological contexts, such as cancer, where SLC3A2 is often overexpressed, promoting tumor growth and therapy resistance.
In biomedical applications, SLC3A2 recombinant protein aids in developing inhibitors targeting amino acid transporters for anticancer therapies. It also serves as a tool to explore integrin-mediated signaling, as CD98hc modulates integrin activation, influencing cell adhesion, migration, and survival. Additionally, its role in immune cell function, including T-cell activation and macrophage polarization, is an emerging area of interest.
Overall, SLC3A2 recombinant protein is a vital resource for dissecting its dual roles in nutrient transport and cell signaling, with implications for understanding diseases and designing targeted therapies.
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