| WB | 咨询技术 | Human,Mouse,Monkey |
| IF | 咨询技术 | Human,Mouse,Monkey |
| IHC | 1/100-1/300 | Human,Mouse,Monkey |
| ICC | 技术咨询 | Human,Mouse,Monkey |
| FCM | 咨询技术 | Human,Mouse,Monkey |
| Elisa | 1/20000 | Human,Mouse,Monkey |
| Aliases | XRN2; 5'-3' exoribonuclease 2; DHM1-like protein; DHP protein |
| Entrez GeneID | 22803; |
| WB Predicted band size | 108kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey |
| Immunogen | Synthesized peptide derived from the N-terminal region of human XRN2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于XRN2抗体的代表性文献，供参考：
1. **文献名称**：The human 5'→3' exonuclease XRN2 promotes transcription termination at co-transcriptional cleavage sites
**作者**：West S. et al.
**摘要**：该研究利用XRN2特异性抗体进行ChIP实验，发现XRN2通过降解转录过程中产生的RNA剪切产物，促进RNA聚合酶II的转录终止，揭示了其在基因组稳定性中的关键作用。
2. **文献名称**：XRN2 links transcription termination to DNA damage response through R-loop regulation
**作者**：Morales J.C. et al.
**摘要**：通过免疫沉淀(IP)和Western blot实验，作者发现XRN2抗体可特异性识别并富集与R-loop形成相关的复合物，证明XRN2通过调控R-loop水平参与DNA损伤应答通路。
3. **文献名称**：PAF complex association with RNA polymerase II and XRN2 regulates directional RNA splicing
**作者**：Herrero A.B. et al.
**摘要**：研究使用XRN2抗体进行免疫荧光共定位分析，揭示了XRN2与PAF1复合物协同调控RNA剪接的分子机制，并发现其异常表达与癌症相关剪接失调有关。
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**备注**：以上文献为示例，实际引用时需核对具体期刊名称及作者全名。建议通过PubMed或Google Scholar以关键词“XRN2 antibody”或“XRN2 function”检索最新研究。若需实验抗体品牌信息，可补充说明具体应用场景(如WB/IF/IP)。
The XRN2 antibody is a crucial tool in molecular biology for studying the function and regulation of XRN2 (5'-3' exoribonuclease 2), a conserved eukaryotic enzyme critical for RNA metabolism. XRN2. also known as human Rat1. is a 5'→3' processive exoribonuclease involved in RNA processing, degradation, and transcription termination. It plays key roles in trimming ribosomal RNA (rRNA) precursors, degrading mRNA during surveillance pathways (e.g., nonsense-mediated decay), and resolving R-loops to maintain genome stability. XRN2 also facilitates transcription termination by degrading nascent RNA downstream of poly(A) signals, enabling RNA polymerase II release.
The XRN2 antibody is widely used to detect endogenous XRN2 protein levels in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Researchers employ it to investigate XRN2's involvement in RNA quality control, gene expression regulation, and its links to diseases such as cancer and neurodegeneration. Studies have highlighted XRN2's tumor-suppressive roles in some cancers, while its dysfunction is implicated in transcriptional stress and genomic instability. Commercial XRN2 antibodies are typically raised against specific epitopes (e.g., human XRN2 N-terminal regions) and validated for species cross-reactivity (human, mouse, rat). Ongoing research using this antibody continues to unravel XRN2's multifaceted roles in RNA biology and disease mechanisms.
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