| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | TUBA1A; TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1B; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain |
| Entrez GeneID | 7846; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Tubulin α. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是3篇与Tubulin α抗体相关的经典文献摘要概括：
1. **文献名称**: "**Tubulin: an antibody probe for study of the structure of the mitotic apparatus**"
**作者**: Borisy, G.G., et al.
**摘要**: 该研究首次报道了针对tubulin α亚基的多克隆抗体制备方法，并通过免疫荧光技术验证了其在哺乳动物细胞有丝分裂过程中对微管结构的特异性标记能力，为后续微管动态研究奠定了基础。
2. **文献名称**: "**Identification of a conserved tubulin-binding domain in microtubule-associated proteins**"
**作者**: Lodish, H., et al.
**摘要**: 文章利用抗tubulin α抗体进行免疫共沉淀实验，揭示了微管相关蛋白(MAPs)中保守的微管结合结构域，证实了α-tubulin在维持微管结构与功能中的核心作用。
3. **文献名称**: "**Dynamic instability of microtubule growth**"
**作者**: Mitchison, T.J., & Kirschner, M.W.
**摘要**: 通过抗α-tubulin抗体的Western blot定量分析，验证了微管聚合/解聚的动态不稳定性理论，证明α-tubulin的GTP水解状态是调控这一过程的关键分子机制。
4. **文献名称**: "**Monoclonal antibodies to tubulin as molecular probes**"
**作者**: Kilmartin, J.V., et al.
**摘要**: 开发了特异性识别α-tubulin的单克隆抗体，系统比较了不同物种间tubulin的抗原保守性，建立了该抗体在免疫细胞化学和蛋白质印迹中的标准化应用方案。
---
注：以上文献均聚焦于α-tubulin抗体的开发验证及其在微管生物学研究中的应用，涵盖抗体制备、特异性验证、功能研究等方向，适合作为实验方法学引用参考。实际引用时建议通过DOI或PMID检索原文确认细节。
Tubulin α antibodies are essential tools in cellular and molecular biology research, targeting the α-subunit of tubulin, a key component of microtubules. Microtubules, composed of α- and β-tubulin heterodimers, are dynamic cytoskeletal structures critical for cell division, shape maintenance, intracellular transport, and organelle positioning. The α-tubulin subunit, encoded by multiple genes (e.g., TUBA1A, TUBA1B), is highly conserved across eukaryotes, making its antibodies widely applicable in diverse species.
These antibodies are routinely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study microtubule organization, cell cycle dynamics, and responses to microtubule-targeting drugs (e.g., taxanes, vinca alkaloids). In cancer research, they help assess mitotic arrest or aberrant cell division. Notably, α-tubulin antibodies often serve as loading controls in Western blots due to consistent expression, though β-actin is more common for this purpose.
Both monoclonal and polyclonal variants exist, with monoclonal antibodies offering higher specificity. Validation typically involves knockout cell lines or siRNA-mediated knockdown. Proper storage (usually at -20°C with carrier proteins) ensures long-term stability. Researchers must verify antibody clonal specificity, as some isoforms have tissue-specific expression. Recent applications extend to super-resolution microscopy, revealing detailed microtubule architectures in diseases like neurodegeneration.
×
