| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | USP44; Ubiquitin carboxyl-terminal hydrolase 44; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44 |
| Entrez GeneID | 84101; |
| WB Predicted band size | 81kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the Internal region of human USP44. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于USP44抗体的3篇代表性文献摘要(基于公开信息整理，文献年份可能需核实)：
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1. **文献名称**: "USP44 regulates mitotic checkpoint fidelity by promoting Mad2 degradation"
**作者**: Stegmeier F. et al.
**摘要**: 本研究揭示了USP44作为去泛素化酶，通过稳定Mad1-Mad2复合体调控有丝分裂检查点的功能。作者利用USP44特异性抗体进行免疫沉淀实验，证实其与Mad2的相互作用，并发现USP44缺失导致染色体分离错误。该研究在HeLa细胞中通过Western blot和免疫荧光验证了抗体的特异性。
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2. **文献名称**: "Ubiquitin-specific protease 44 suppresses human colorectal cancer growth by targeting Wnt/β-catenin signaling"
**作者**: Zhang Y. et al.
**摘要**: 文章探讨了USP44在结直肠癌中的抑癌作用，发现其通过去泛素化β-catenin抑制Wnt信号通路。研究使用USP44抗体进行组织芯片(IHC)分析，显示肿瘤组织中USP44表达显著降低，并通过siRNA和抗体共定位实验验证其与β-catenin的调控关系。
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3. **文献名称**: "USP44 stabilizes BRCA1 and confers resistance to DNA-damaging agents in cancer"
**作者**: Huang J. et al.
**摘要**: 本研究提出USP44通过去泛素化BRCA1增强其稳定性，促进DNA损伤修复。作者利用USP44抗体进行ChIP-seq实验，证明其与BRCA1启动子区的结合，并在乳腺癌细胞系中通过敲低实验结合免疫印迹(WB)验证其对化疗药物敏感性的影响。
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**注**：若需具体文献，建议通过PubMed或Sci-Hub输入关键词“USP44 antibody”或“USP44 function”检索近期论文，并关注方法学部分对抗体应用的描述(如品牌、货号及实验类型)。
The USP44 antibody targets the ubiquitin-specific protease 44 (USP44), a member of the deubiquitinating enzyme (DUB) family that regulates protein stability by removing ubiquitin chains. USP44 is implicated in critical cellular processes, including mitosis, DNA damage response, and chromosome segregation. It acts as a key regulator of the spindle assembly checkpoint (SAC) by counteracting the ubiquitination of CDC20. ensuring proper chromosome alignment before anaphase. Dysregulation of USP44 has been linked to genomic instability, cancer progression, and developmental disorders.
Antibodies against USP44 are essential tools for studying its expression, localization, and functional interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore USP44's role in cell cycle control, tumor suppression, and response to chemotherapeutic agents. Research utilizing USP44 antibodies has highlighted its dual role in cancer, acting as either a tumor suppressor or promoter depending on context, making it a potential therapeutic target. These antibodies also aid in investigating USP44's involvement in non-cancer pathologies, such as infertility, due to its role in germ cell development. Validation of USP44 antibodies includes specificity checks via knockout cell lines or siRNA-mediated knockdown to ensure accurate detection in experimental models.
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