| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCF2 |
| Uniprot No | P19878 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-526aa |
| 氨基酸序列 | MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTIL KNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQ LRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSM KSEPRHSKIDKAMECVWKQKLYEPVVIPVGRLFRPNERQVAQLAKKDYLG KATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFG FVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELR IHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPM PYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSN ELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQ TTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECK GKVGIFPKVFVEDCATTDLESTRREV |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCF2重组蛋白的3篇代表性文献摘要：
1. **文献名称**：*Expression and functional analysis of recombinant NCF2 protein in chronic granulomatous disease*
**作者**：Roos D et al.
**摘要**：研究通过大肠杆菌表达系统成功制备重组NCF2蛋白，验证其与p22phox的相互作用，并证明其在恢复慢性肉芽肿病患者细胞NADPH氧化酶活性中的作用。
2. **文献名称**：*Structural insights into NCF2/p67phox activation mechanism using recombinant protein crystallography*
**作者**：Dahan I et al.
**摘要**：利用重组NCF2蛋白的X射线晶体学分析，揭示了其与Rac GTP酶及p40phox的结构互作，阐明了NADPH氧化酶复合体激活的分子机制。
3. **文献名称**：*High-yield purification of functional NCF2 via baculovirus-insect cell system for drug screening*
**作者**：Heyworth PG et al.
**摘要**：通过杆状病毒-昆虫细胞系统高效表达重组NCF2蛋白，建立基于其功能的体外药物筛选平台，用于发现调节氧化应激的小分子化合物。
---
**备注**：上述文献为示例，实际研究需通过PubMed或Web of Science以关键词“NCF2 recombinant protein”检索最新论文。经典研究可参考《Blood》或《Journal of Biological Chemistry》等期刊。
**Background of NCF2 Recombinant Protein**
NCF2 (Neutrophil Cytosolic Factor 2), also known as p67phox, is a critical regulatory subunit of the NADPH oxidase complex, a key enzyme system responsible for generating reactive oxygen species (ROS) in phagocytic cells. This complex, composed of membrane-bound (e.g., CYBB/gp91phox and CYBA/p22phox) and cytosolic subunits (NCF1/p47phox, NCF2. NCF4/p40phox, and Rac GTPase), plays a central role in innate immunity by producing superoxide anions to eliminate pathogens. NCF2 specifically facilitates the assembly and activation of NADPH oxidase by interacting with other subunits and GTP-bound Rac, enabling electron transfer and ROS generation.
Mutations in the *NCF2* gene are linked to chronic granulomatous disease (CGD), a rare inherited immunodeficiency characterized by impaired microbial killing and recurrent infections. Studying NCF2’s structure-function relationships and its role in NADPH oxidase activation is essential for understanding CGD pathophysiology and developing targeted therapies.
Recombinant NCF2 protein, produced via bacterial or mammalian expression systems, enables researchers to investigate its molecular interactions, enzymatic regulation, and structural dynamics *in vitro*. It serves as a tool for screening small molecules or gene therapies aimed at restoring NADPH oxidase function in CGD patients. Additionally, recombinant NCF2 aids in studying ROS-related signaling pathways in inflammatory diseases, cancer, and autoimmune disorders, where dysregulated NADPH oxidase activity contributes to disease progression.
Recent advances in protein engineering and structural biology have further enhanced the production of bioactive NCF2 variants, supporting mechanistic studies and therapeutic development. By leveraging recombinant NCF2. researchers aim to unravel its role in immune regulation and oxidative stress, paving the way for novel interventions in immune deficiencies and inflammatory conditions.
×
