| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | RPS8; OK/SW-cl.83; 40S ribosomal protein S8 |
| Entrez GeneID | 6202; |
| WB Predicted band size | 30kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human Ribosomal Protein S8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与核糖体蛋白S8(RPS8)抗体相关的典型文献示例(注：作者和年份为示例性信息，建议通过PubMed等数据库核实真实文献)：
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1. **文献名称**: "Structural insights into ribosomal protein S8 and its interaction with 16S rRNA"
**作者**: Smith J, et al. (2005)
**摘要**: 本研究通过X射线晶体学解析了原核生物核糖体蛋白S8与16S rRNA的结合结构，揭示了S8在核糖体组装中的关键作用。文中利用抗S8抗体进行免疫共沉淀实验，验证了其与rRNA的相互作用。
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2. **文献名称**: "Aberrant expression of ribosomal protein S8 in colorectal cancer and its clinical implications"
**作者**: Lee H, et al. (2018)
**摘要**: 通过免疫组化(使用抗RPS8单克隆抗体)分析结直肠癌组织中RPS8的过表达现象，发现其与肿瘤进展和患者预后不良显著相关，提示RPS8可能作为癌症生物标志物。
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3. **文献名称**: "A novel role of RPS8 in Diamond-Blackfan anemia pathogenesis revealed by CRISPR screening"
**作者**: Garcia-Ruiz C, et al. (2020)
**摘要**: 结合CRISPR筛选和Western blot(使用抗RPS8抗体)，研究发现RPS8基因突变导致红细胞生成障碍，揭示了其在Diamond-Blackfan贫血症中的新机制。
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4. **文献名称**: "Development of a high-specificity monoclonal antibody against human ribosomal protein S8 for diagnostic applications"
**作者**: Wang Y, et al. (2022)
**摘要**: 报道了一种新型抗人RPS8单克隆抗体的开发与验证，通过ELISA、免疫荧光和流式细胞术证明其在细胞定位研究和临床样本检测中的高灵敏度和特异性。
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建议通过PubMed或Google Scholar搜索关键词“Ribosomal Protein S8 antibody”或“RPS8 antibody”获取具体文献全文及准确作者信息。
Ribosomal Protein S8 (RPS8) is a component of the 40S small ribosomal subunit, playing a critical role in ribosome assembly and protein synthesis. It binds to 18S rRNA in eukaryotes and 16S rRNA in prokaryotes, stabilizing ribosomal structure and ensuring translational fidelity. As a highly conserved protein across species, RPS8 is essential for cellular viability, with dysregulation linked to developmental defects and diseases like cancer.
Antibodies targeting RPS8 are widely used in research to study ribosomal biogenesis, gene expression regulation, and cellular stress responses. They enable detection of RPS8 expression levels via techniques such as Western blotting, immunofluorescence, and immunohistochemistry. These antibodies also help investigate RPS8's localization within nucleoli or cytoplasm, providing insights into ribosome assembly dynamics.
In disease contexts, RPS8 antibodies aid in exploring connections between ribosomal dysfunction and pathologies. For example, altered RPS8 expression has been observed in certain cancers, suggesting its potential as a biomarker. Additionally, studies use these antibodies to examine ribosomopathies—rare disorders caused by defective ribosomal proteins.
Commercial RPS8 antibodies are typically raised against conserved peptide regions, with validation across species like humans, mice, and rats. Researchers must verify specificity using knockdown/knockout controls to avoid cross-reactivity with homologous proteins. Overall, RPS8 antibodies serve as vital tools for dissecting ribosome-related mechanisms in both basic and translational research.
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