| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD96 |
| Uniprot No | P40200-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-503aa |
| 氨基酸序列 | VWEKTVNTEE NVYATLGSDV NLTCQTQTVG FFVQMQWSKV TNKIDLIAVY HPQYGFYCAY GRPCESLVTF TETPENGSKW TLHLRNMSCS VSGRYECMLV LYPEGIQTKI YNLLIQTHVT ADEWNSNHTI EIEINQTLEI PCFQNSSSKI SSEFTYAWSV EDNGTQETLI SQNHLISNST LLKDRVKLGT DYRLHLSPVQ IFDDGRKFSC HIRVGPNKIL RSSTTVKVFA KPEIPVIVEN NSTDVLVERR FTCLLKNVFP KANITWFIDG SFLHDEKEGI YITNEERKGK DGFLELKSVL TRVHSNKPAQ SDNLTIWCMA LSPVPGNKVW NISSEKITFL LGSEISSTDP PLSVTESTLD TQPSPASSVS PARYPATSSV TLVDVSALRP NTTPQPSNSS MTTRGFNYPW TSSGTDTKKS VSRIPSETYS SSPSGAGSTL HDNVFTSTAR AFSEVPTTAN GSTKTNHVHI TGIVVNKPKD GMLEHHHHHH |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CD96重组蛋白的参考文献概览：
1. **文献名称**: "CD96 Interaction with ADAM10 in T Cell Differentiation and Tumor Immunity"
**作者**: Wang Y, et al. (2021)
**摘要**: 本研究通过重组CD96蛋白与ADAM10的体外结合实验，揭示CD96通过调控T细胞分化影响肿瘤免疫微环境的分子机制，为靶向CD96的免疫疗法提供依据。
2. **文献名称**: "Structural characterization of CD96-Fc fusion protein and its role in NK cell exhaustion"
**作者**: Zhang L, et al. (2019)
**摘要**: 作者利用昆虫细胞系统表达CD96-Fc重组蛋白，解析其晶体结构，并证明该蛋白可逆转NK细胞在慢性病毒感染中的功能耗竭，提出其作为免疫检查点抑制剂的潜力。
3. **文献名称**: "Recombinant CD96 extracellular domain inhibits melanoma metastasis in murine models"
**作者**: Tanaka K, et al. (2020)
**摘要**: 该研究在大肠杆菌中表达可溶性CD96重组蛋白，通过小鼠黑色素瘤模型证实其阻断CD96与配体结合，显著抑制肿瘤转移并增强CD8+ T细胞浸润。
注：以上文献信息为模拟创作，实际文献需通过PubMed或Web of Science检索确认。建议使用关键词 "CD96 recombinant protein" + "function"/"structure"/"cancer" 查找最新实证研究。
CD96 (also known as TACTILE) is a cell surface protein belonging to the immunoglobulin superfamily, primarily expressed on natural killer (NK) cells, activated T cells, and certain subsets of lymphocytes. It functions as an immune checkpoint receptor, interacting with ligands such as CD155 (PVR) to modulate immune responses, particularly in tumor microenvironments. CD96 has garnered attention for its dual role in both promoting and suppressing immune activity, depending on cellular context and signaling dynamics. Structurally, it consists of extracellular immunoglobulin domains, a transmembrane region, and a cytoplasmic tail containing tyrosine phosphorylation motifs that mediate intracellular signaling.
Recombinant CD96 proteins are engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to study its biological functions, ligand interactions, and therapeutic potential. These proteins retain key functional domains, enabling researchers to investigate CD96's role in immune evasion by tumors, where its engagement with CD155 often dampens NK/T cell cytotoxicity. Preclinical studies suggest that blocking CD96-CD155 interactions enhances antitumor immunity, positioning CD96 as a candidate target for cancer immunotherapy. Additionally, recombinant CD96 aids in developing diagnostic tools or bispecific antibodies to fine-tune immune responses.
Despite its promise, CD96's complex biology—such as competition with the activating receptor CD226 for CD155 binding—requires further exploration. Current research focuses on delineating its signaling pathways and evaluating therapeutic strategies, including combination therapies with PD-1/CTLA-4 inhibitors. Recombinant CD96 proteins thus serve as critical tools to decode its immunoregulatory mechanisms and advance translational applications in oncology and autoimmune diseases.
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