| 纯度 | >95%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | REG3B |
| Uniprot No | P35230 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-175aa |
| 氨基酸序列 | MLPPTACSVM SWMLLSCLML LSQVQGEDSL KNIPSARISC PKGSQAYGSY CYALFQIPQT WFDAELACQK RPGGHLVSVL NSAEASFLSS MVKRTGNSYQ YTWIGLHDPT LGAEPNGGGW EWSNNDVMNY FNWERNPSTA LDRAFCGSLS RASGFLKWRD MTCEVKLPYV CKFTG |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REG3B重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*REG3B promotes cell proliferation and suppresses apoptosis in pancreatic cancer through p38 signaling*
**作者**：Li et al. (2021)
**摘要**：研究发现重组REG3B蛋白通过激活p38 MAPK信号通路，促进胰腺癌细胞增殖并抑制细胞凋亡，提示其在肿瘤进展中的潜在作用。
2. **文献名称**：*Recombinant REG3B protein enhances intestinal barrier function via modulating tight junctions*
**作者**：Wang et al. (2019)
**摘要**：通过体外实验证明，重组REG3B蛋白可上调肠上皮细胞紧密连接蛋白表达，改善脂多糖诱导的肠道屏障损伤，具有修复黏膜屏障的潜力。
3. **文献名称**：*Antimicrobial activity of recombinant human REG3B against Gram-positive bacteria in chronic inflammation*
**作者**：Mukherjee et al. (2017)
**摘要**：重组人源REG3B蛋白表现出对金黄色葡萄球菌等革兰氏阳性菌的选择性杀菌活性，揭示了其在慢性炎症中作为宿主防御肽的机制。
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注：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science检索最新文献。REG3B研究多聚焦于其在癌症、肠道疾病及抗菌免疫中的双重功能。
REG3B, also known as regenerating islet-derived protein 3-beta (PAP IB or HIP/PAP), is a secreted protein belonging to the REG (regenerating gene) family, which is implicated in cell proliferation, differentiation, and tissue repair. This protein is primarily expressed in the gastrointestinal tract, particularly in Paneth cells and intestinal epithelial cells, where it plays a critical role in maintaining mucosal homeostasis and host-microbe interactions. Structurally, REG3B contains a conserved C-type lectin domain, enabling its function as a bactericidal agent against Gram-positive bacteria by binding to peptidoglycan components on microbial surfaces.
Physiologically, REG3B is upregulated during intestinal inflammation, infection, or injury, acting as a key mediator in innate immunity. It contributes to the containment of gut microbiota, preventing bacterial translocation across the epithelial barrier. Studies also suggest its involvement in promoting epithelial cell survival, wound healing, and anti-apoptotic signaling, highlighting its dual role in microbial defense and tissue regeneration. Dysregulation of REG3B has been linked to pathologies such as inflammatory bowel disease (IBD), pancreatitis, and cancers. For instance, elevated REG3B levels are observed in hepatocellular carcinoma and pancreatic ductal adenocarcinoma, where it may facilitate tumor progression and metastasis.
Recombinant REG3B protein, produced via bacterial or mammalian expression systems, is widely used to investigate its biological functions and therapeutic potential. Preclinical models demonstrate its capacity to enhance mucosal repair and mitigate bacterial infection, sparking interest in its application for treating gastrointestinal disorders. However, its pro-tumorigenic effects in certain contexts underscore the need for nuanced research. Current studies focus on elucidating its signaling pathways, receptor interactions, and tissue-specific roles to harness its regenerative properties while minimizing oncogenic risks.
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