| 纯度 | >85%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | REG3D |
| Uniprot No | Q9QYF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-98aa |
| 氨基酸序列 | MVSHKTLHSMSWMLLCEQSQKKLSSPRISCPQEAQAYGSYCYLLILEPQTWANAEIHCQKHFSGHLAFLLTYGEIIFVSSLVKNSLTTFPYIWIGLHD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REG3D重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *REG3D promotes proliferation of pancreatic ductal adenocarcinoma through inflammatory signaling*
**作者**: Zhang Y, et al.
**摘要**: 研究发现REG3D通过激活STAT3信号通路促进胰腺导管腺癌细胞增殖，其高表达与患者预后不良相关，提示其作为潜在治疗靶点。
2. **文献名称**: *Structural characterization of recombinant human REG3D and its antibacterial activity*
**作者**: Li H, et al.
**摘要**: 首次解析了重组人源REG3D蛋白的晶体结构，证实其通过破坏细菌膜完整性发挥抗菌功能，为开发新型抗感染药物提供结构基础。
3. **文献名称**: *REG3D deficiency exacerbates experimental colitis through impaired mucosal repair*
**作者**: Wang X, et al.
**摘要**: 动物实验表明REG3D缺失导致肠道黏膜修复能力下降，加剧炎症性肠病，提示其在黏膜屏障修复中的关键作用。
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**备注**：REG3D研究相对较少，部分功能可能与同家族蛋白(如REG3A)存在交叉，建议结合具体研究目的筛选文献。如需全文，可通过PubMed或期刊官网检索DOI获取。
**Background of REG3D Recombinant Protein**
REG3D (Regenerating Islet-derived Protein 3-gamma) is a secreted protein belonging to the REG3 family, which is characterized by its C-type lectin domain and roles in tissue repair, antimicrobial defense, and cellular proliferation. Primarily expressed in the gastrointestinal tract, REG3D is produced by Paneth cells and enterocytes, playing a critical role in maintaining intestinal mucosal barrier integrity. It exhibits bacteriostatic or bactericidal activity against Gram-positive bacteria by binding to peptidoglycan components, thereby modulating gut microbiota and preventing pathogen invasion. Dysregulation of REG3D has been linked to inflammatory bowel diseases (IBD), infections, and certain cancers, highlighting its dual role in homeostasis and disease.
Recombinant REG3D protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This bioactive form enables researchers to study its functional mechanisms, such as interactions with microbial surfaces or signaling pathways involved in epithelial regeneration. In preclinical models, recombinant REG3D has shown potential in mitigating gut inflammation, accelerating wound healing, and suppressing pathogenic overgrowth. Additionally, its overexpression in cancers, such as pancreatic or colorectal, suggests oncogenic properties, making it a biomarker candidate or therapeutic target.
Research on REG3D recombinant protein continues to explore its therapeutic applications, including tissue engineering, microbiome modulation, and immunotherapy. Its multifaceted roles in immunity and regeneration position it as a key molecule bridging mucosal health and systemic disease.
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