| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAM15 |
| Uniprot No | Q13444-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-696aa |
| 氨基酸序列 | MRLALLWALG LLGAGSPLPS WPLPNIGGTE EQQAESEKAP REPLEPQVLQ DDLPISLKKV LQTSLPEPLR IKLELDGDSH ILELLQNREL VPGRPTLVWY QPDGTRVVSE GHTLENCCYQ GRVRGYAGSW VSICTCSGLR GLVVLTPERS YTLEQGPGDL QGPPIISRIQ DLHLPGHTCA LSWRESVHTQ KPPEHPLGQR HIRRRRDVVT ETKTVELVIV ADHSEAQKYR DFQHLLNRTL EVALLLDTFF RPLNVRVALV GLEAWTQRDL VEISPNPAVT LENFLHWRRA HLLPRLPHDS AQLVTGTSFS GPTVGMAIQN SICSPDFSGG VNMDHSTSIL GVASSIAHEL GHSLGLDHDL PGNSCPCPGP APAKTCIMEA STDFLPGLNF SNCSRRALEK ALLDGMGSCL FERLPSLPPM AAFCGNMFVE PGEQCDCGFL DDCVDPCCDS LTCQLRPGAQ CASDGPCCQN CQLRPSGWQC RPTRGDCDLP EFCPGDSSQC PPDVSLGDGE PCAGGQAVCM HGRCASYAQQ CQSLWGPGAQ PAAPLCLQTA NTRGNAFGSC GRNPSGSYVS CTPRDAICGQ LQCQTGRTQP LLGSIRDLLW ETIDVNGTEL NCSWVHLDLG SDVAQPLLTL PGTACGPGLV CIDHRCQRVD LLGAQECRSK CHGHGVCDSN RHCYCEEGWA PPDCTTQLKA TSSLTT |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADAM15重组蛋白的3篇模拟参考文献，内容基于该领域常见研究方向概括：
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1. **文献名称**：*ADAM15重组蛋白在肿瘤细胞侵袭中的作用机制研究*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌表达系统重组ADAM15胞外域蛋白，发现其能增强肿瘤细胞的迁移与侵袭能力，并证实该过程与整合素信号通路的激活相关，提示ADAM15可能成为癌症治疗的潜在靶点。
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2. **文献名称**：*ADAM15重组蛋白的酶活性及其在炎症中的调控*
**作者**：Zhang L, et al.
**摘要**：作者利用哺乳动物细胞表达纯化ADAM15全长重组蛋白，证实其具有金属蛋白酶活性，可剪切细胞外基质蛋白如纤维连接蛋白，并促进炎症因子释放，为ADAM15在类风湿性关节炎中的作用提供了实验依据。
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3. **文献名称**：*ADAM15重组蛋白与血管生成的功能关联*
**作者**：Wang H, et al.
**摘要**：通过昆虫细胞表达系统获得高纯度ADAM15重组蛋白，研究发现其通过调控VEGF受体2的胞内信号传导，促进内皮细胞管腔形成，揭示了ADAM15在病理性血管生成中的关键角色。
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**注**：以上文献为模拟内容，实际研究中建议通过PubMed、Web of Science等数据库检索真实发表文献。
ADAM15 (A Disintegrin and Metalloproteinase 15) is a member of the ADAM family of transmembrane proteins, which are characterized by their multidomain structure combining metalloproteinase, disintegrin, and cell-adhesion functionalities. These proteins play critical roles in cell-cell and cell-matrix interactions, proteolytic processing of signaling molecules, and modulation of cellular signaling pathways. ADAM15. in particular, is composed of a prodomain, metalloproteinase domain, disintegrin-like domain, cysteine-rich region, transmembrane domain, and cytoplasmic tail. While its metalloproteinase activity is relatively weak compared to other ADAMs, ADAM15 is notable for its disintegrin domain, which mediates interactions with integrins, influencing cell adhesion, migration, and intracellular signaling.
First identified in the 1990s, ADAM15 is expressed in various tissues, including endothelial cells, smooth muscle cells, and immune cells, and is upregulated in pathological conditions such as cancer, inflammation, and cardiovascular diseases. In cancer, ADAM15 contributes to tumor progression by promoting cell invasion, angiogenesis, and metastasis through proteolytic cleavage of extracellular matrix components or growth factors (e.g., VEGF, EGF). It also modulates inflammatory responses by shedding cytokines (e.g., TNF-α) or regulating leukocyte adhesion.
Recombinant ADAM15 protein, produced via expression systems like mammalian or insect cells, enables researchers to study its structure-function relationships, substrate specificity, and interactions with therapeutic agents. Its applications span mechanistic studies in disease models, drug discovery (e.g., inhibitors targeting metalloproteinase or disintegrin domains), and diagnostic biomarker development. Despite its complex roles, ADAM15 remains a compelling therapeutic target due to its dual involvement in pathological and protective processes, depending on cellular context. Ongoing research aims to unravel its context-dependent mechanisms and therapeutic potential.
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