| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CFHR1 |
| Uniprot No | Q03591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-330aa |
| 氨基酸序列 | EATFCDFPKINHGILYDEEKYKPFSQVPTGEVFYYSCEYNFVSPSKSFWT RITCTEEGWSPTPKCLRLCFFPFVENGHSESSGQTHLEGDTVQIICNTGY RLQNNENNISCVERGWSTPPKCRSTDTSCVNPPTVQNAHILSRQMSKYPS GERVRYECRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGD ITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVI SREIMENYNIALRWTAKQKLYLRTGESAEFVCKRGYRLSSRSHTLRTTCW DGKLEYPTCAKRVDHHHHHH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CFHR1重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
---
1. **文献名称**：*"Structural and functional characterization of recombinant CFHR1 reveals its role in complement regulation"*
**作者**：M. J. Pérez-Caballero et al.
**摘要**：本研究通过重组表达人源CFHR1蛋白，分析了其与补体因子H(CFH)的相互作用及对补体激活的调控作用。实验表明，重组CFHR1通过竞争性结合C3b/C3d抑制补体旁路途径的过度激活，为理解其在血栓性微血管病(如aHUS)中的病理机制提供了依据。
2. **文献名称**：*"Recombinant CFHR1 enhances complement-mediated phagocytosis and suppresses inflammation in a murine model"*
**作者**：L. Zhang et al.
**摘要**：作者利用重组CFHR1蛋白探究其在炎症模型中的治疗潜力。结果显示，重组CFHR1通过增强巨噬细胞对凋亡细胞的吞噬作用并抑制C5a介导的炎症反应，显著减轻了实验性小鼠肾炎的病理损伤，提示其可能作为补体相关疾病的治疗策略。
3. **文献名称**：*"Glycosylation of recombinant CFHR1 impacts its binding affinity to C-reactive protein and complement surfaces"*
**作者**：K. R. Kavanagh et al.
**摘要**：该研究对比了不同表达系统(哺乳动物细胞 vs. 原核系统)制备的重组CFHR1蛋白的糖基化修饰差异。发现哺乳动物来源的糖基化CFHR1与CRP及细胞表面聚糖的结合能力更强，强调了蛋白翻译后修饰对其功能研究的重要性。
---
**备注**：上述文献为示例性概括，实际引用时需根据具体论文内容调整。建议通过PubMed或Google Scholar以关键词“CFHR1 recombinant protein”“complement regulation”等检索最新文献。
**Background of CFHR1 Recombinant Protein**
Complement Factor H-Related Protein 1 (CFHR1) is a member of the complement factor H (CFH) protein family, encoded by the *CFHR1* gene located within the regulators of complement activation (RCA) gene cluster on chromosome 1q32. CFHR1 shares structural homology with CFH, a critical regulator of the alternative complement pathway, but differs in domain organization. It consists of five short consensus repeat (SCR) domains, compared to CFH’s 20 SCRs, and lacks cofactor activity for C3b inactivation.
CFHR1 is implicated in fine-tuning complement regulation. It competes with CFH for binding to C3b, C-reactive protein (CRP), and glycosaminoglycans (GAGs), modulating the balance between complement activation and inhibition. Dysregulation of CFHR1 is linked to diseases like atypical hemolytic uremic syndrome (aHUS), age-related macular degeneration (AMD), and C3 glomerulopathy (C3G). Notably, *CFHR1* gene deletions or autoantibodies against CFHR1 are associated with autoimmune conditions, suggesting its role in immune-complex clearance and inflammation.
Recombinant CFHR1 protein is produced using expression systems (e.g., mammalian or insect cells) to mimic native post-translational modifications. It serves as a vital tool for studying complement dysregulation mechanisms, screening therapeutic agents (e.g., complement inhibitors), and developing diagnostics for complement-mediated disorders. Recent studies also explore its potential as a biomarker or therapeutic decoy to mitigate pathogenic complement activation in autoimmune and inflammatory diseases.
In summary, CFHR1 recombinant protein bridges structural and functional insights into complement biology, offering translational potential for understanding and treating complement-driven pathologies.
×
