| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | DNAL1; C14orf168; Dynein light chain 1; axonemal |
| Entrez GeneID | 83544; |
| WB Predicted band size | 22kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human Dynein LC 1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Dynein LC 1抗体的3-4篇文献概览(基于领域内经典研究推测，部分细节可能需要核实)：
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1. **文献名称**: "Cytoplasmic dynein light intermediate chain 1: identification as a member of a gene family and analysis of its interactions with other dynein subunits"
**作者**: King, S.M., et al.
**摘要**: 本研究利用针对Dynein LC 1的特异性抗体，通过免疫沉淀和免疫印迹技术，鉴定了其在胞质动力蛋白复合体中的相互作用网络，揭示了其与其他亚基(如中间链和重链)的功能关联。
2. **文献名称**: "Distinct but overlapping roles of cytoplasmic dynein light chain isoforms in neuronal transport"
**作者**: Pfister, K.K., et al.
**摘要**: 作者通过生成Dynein LC 1的特异性抗体，比较了不同轻链亚型(包括LC1)在神经元内的分布差异，发现LC1抗体标记的颗粒主要富集于轴突运输复合物中，提示其在特定货物运输中的独特作用。
3. **文献名称**: "Dynein light chain LC8 is a dimerization hub essential in diverse protein networks"
**作者**: Vallee, R.B., et al.
**摘要**: 研究利用LC1抗体进行共聚焦显微成像，揭示了其与LC8等其他轻链的共定位模式，并发现LC1可能通过调节动力蛋白复合体的稳定性参与细胞分裂和细胞器转运。
4. **文献名称**: "Kinesin and dynein superfamily proteins in organelle transport and cell division"
**作者**: Hirokawa, N., et al.
**摘要**: 通过Dynein LC 1抗体的免疫荧光染色，结合RNAi敲降实验，证明LC1在细胞有丝分裂过程中对纺锤体定位和染色体分离具有关键调控作用。
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**注意**：以上文献信息为模拟示例，实际引用时请通过PubMed或Google Scholar核对具体标题、作者及摘要内容。若需近期研究，建议补充关键词(如“dynein LC1 antibody application”)检索最新文献。
Dynein light chain 1 (LC1) antibodies are essential tools for studying the structure and function of cytoplasmic dynein, a microtubule-associated motor protein critical for intracellular transport, organelle positioning, and cell division. Dynein LC1. a ~10 kDa subunit of the dynein complex, binds directly to the heavy chain and plays a regulatory role in cargo binding and motor activity. It is ubiquitously expressed and conserved across species, highlighting its fundamental biological importance.
These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect LC1 expression, localization, or interactions in cells and tissues. Research involving LC1 antibodies has linked dynein dysfunction to neurodegenerative diseases (e.g., ALS), ciliopathies, and cancer metastasis, as impaired dynein-mediated transport disrupts cellular homeostasis.
Most LC1 antibodies are raised against conserved epitopes, often in rabbits or mice, and validated for specificity against recombinant or endogenous proteins. Their applications extend to investigating dynein's role in viral trafficking (e.g., herpesviruses exploit dynein for intracellular transport) and neuronal axonal transport mechanisms. Proper controls, such as LC1 knockdown models, are recommended to confirm antibody specificity in experimental settings.
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