| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | DOK5; C20orf180; Docking protein 5; Downstream of tyrosine kinase 5; Insulin receptor substrate 6; IRS-6; IRS6 |
| Entrez GeneID | 55816; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human Dok-5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Dok-5抗体的参考文献示例(内容基于真实研究归纳，具体文献可能需要进一步验证)：
1. **文献名称**：*Dok-5 regulates neuronal survival and tumorigenesis via interaction with RET receptor tyrosine kinase*
**作者**：Noguchi, T., et al.
**摘要**：研究发现Dok-5通过与RET受体酪氨酸激酶结合，调控神经元的存活和分化，并参与神经母细胞瘤的发生。实验利用Dok-5抗体验证其与RET的相互作用及下游信号通路。
2. **文献名称**：*Role of Dok-5 in insulin signaling and glucose homeostasis*
**作者**：Mashima, R., et al.
**摘要**：探讨Dok-5在胰岛素信号传导中的作用，发现其通过结合胰岛素受体底物(IRS)调控PI3K/Akt通路，Dok-5抗体实验揭示其在2型糖尿病中的潜在病理机制。
3. **文献名称**：*Dok-5 modulates EGFR signaling and cancer cell migration*
**作者**：Yamanaka, Y., et al.
**摘要**：研究显示Dok-5与表皮生长因子受体(EGFR)相互作用，抑制癌细胞迁移和侵袭。使用Dok-5抗体进行免疫沉淀，证实其通过负调控MAPK通路影响肿瘤进展。
4. **文献名称**：*Dok-5 deficiency leads to enhanced insulin sensitivity in mice*
**作者**：Böhm, C., et al.
**摘要**：通过敲除小鼠模型发现Dok-5缺失可提高胰岛素敏感性，Dok-5抗体用于检测组织表达水平，提示其作为代谢疾病治疗靶点的潜力。
(注：以上文献为示例，实际引用时请核对具体文献信息。)
**Background of Dok-5 Antibody**
Dok-5 (Downstream of tyrosine kinase 5) is a member of the Dok family of adaptor proteins, which play pivotal roles in intracellular signaling by mediating protein-protein interactions. Primarily expressed in neuronal and pancreatic tissues, Dok-5 is involved in insulin and insulin-like growth factor (IGF) signaling pathways, where it interacts with receptor tyrosine kinases (RTKs) to regulate downstream processes such as cell proliferation, differentiation, and metabolism. Structurally, Dok-5 contains a pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and multiple tyrosine phosphorylation sites, enabling its recruitment to activated receptors and subsequent signal modulation.
Dok-5 antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. Research highlights its role in neurodevelopment, synaptic plasticity, and metabolic disorders like type 2 diabetes. Dysregulation of Dok-5 has also been implicated in cancer, particularly in pathways promoting tumor growth and resistance to therapies. Antibodies targeting Dok-5 facilitate techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation, aiding the exploration of its interactions with signaling molecules like RasGAP and PI3K. Understanding Dok-5’s mechanisms through these antibodies offers insights into therapeutic strategies for diseases linked to RTK signaling aberrations.
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