| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGO3 |
| Uniprot No | Q9H9G7-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 氨基酸序列 | MCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCN VTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQE QKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLV RSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHG VWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGM PIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVK RVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQR PSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQR PRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYE LLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAG TTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLT YQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQ SNGRDPQALAKAVQIHQDTLRTMYFA |
| 预测分子量 | 98 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGO3重组蛋白的3篇参考文献概括：
1. **文献名称**：*Structural insights into the human Argonaute-3 protein and its role in RNA silencing*
**作者**：Smith J. et al.
**摘要**：通过重组表达系统纯化人源AGO3蛋白，解析其晶体结构，揭示其与siRNA/miRNA结合的独特构象，并发现其与其他Argonaute家族成员(如AGO2)在催化活性上的差异，提示AGO3可能通过非切割机制参与基因沉默。
2. **文献名称**：*Functional characterization of recombinant AGO3 in Drosophila germline development*
**作者**：Tanaka M. et al.
**摘要**：利用重组果蝇AGO3蛋白进行体外结合实验，证明其与piRNA(PIWI互作RNA)特异性结合，并通过调控转座子RNA的稳定性参与生殖细胞发育和基因组稳定性维持。
3. **文献名称**：*Recombinant AGO3 exhibits antiviral activity in mammalian cells*
**作者**：Lee S. et al.
**摘要**：研究显示，重组表达的AGO3蛋白可通过结合病毒衍生的小RNA，抑制特定RNA病毒(如丙型肝炎病毒)的复制，为基于Argonaute蛋白的抗病毒治疗提供新思路。
注：上述文献为示例性概括，实际研究中请根据具体需求检索并引用正式发表的论文。
**Background of AGO3 Recombinant Protein**
AGO3 (Argonaute 3) is a member of the Argonaute protein family, which plays a central role in RNA-mediated gene silencing pathways, including the RNA interference (RNAi) and microRNA (miRNA) regulatory mechanisms. These evolutionarily conserved proteins are critical for gene expression regulation, genome stability, and antiviral defense. Structurally, AGO proteins contain two conserved domains: the PAZ domain, which binds small RNA molecules, and the PIWI domain, which often exhibits endonuclease activity (in some family members like AGO2). While AGO2 is the most studied due to its catalytic "slicer" function, AGO3 is particularly notable in *Drosophila melanogaster*, where it contributes to the amplification of the RNAi response through the "ping-pong" mechanism in conjunction with Piwi proteins.
Recombinant AGO3 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., insect or mammalian cells) to ensure proper folding and post-translational modifications. The purified protein retains its ability to bind small RNAs and participate in RNA-induced silencing complexes (RISCs), making it a valuable tool for studying RNA-guided gene regulation. Researchers use recombinant AGO3 to dissect its role in antiviral immunity, transposon suppression, and epigenetic modifications. Additionally, it aids in exploring functional differences among Argonaute paralogs, particularly in models lacking endogenous AGO3 expression.
In biomedical research, AGO3 recombinant protein contributes to understanding diseases linked to RNA dysregulation, such as cancer and neurodegeneration. Its applications extend to *in vitro* assays for drug screening and mechanistic studies of gene silencing pathways. Despite its lower catalytic activity compared to AGO2. AGO3 remains a key subject for unraveling the complexity of RNA-mediated cellular processes.
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