关键信息
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基因名
DNASEII
- 应用
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别名
DNASEII；DNASE2A；DNL2；Deoxyribonuclease-2-alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00115
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表达区间
1-360aa
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氨基酸序列
MIPLLLAALLCVPAGALTCYGDSGQPVDWFVVYKLPALRGSGEAAQRGLQ YKYLDESSGGWRDGRALINSPEGAVGRSLQPLYRSNTSQLAFLLYNDQPP QPSKAQDSSMRGHTKGVLLLDHDGGFWLVHSVPNFPPPASSAAYSWPHSA CTYGQTLLCVSFPFAQFSKMGKQLTYTYPWVYNYQLEGIFAQEFPDLENV VKGHHVSQEPWNSSITLTSQAGAVFQSFAKFSKFGDDLYSGWLAAALGTN LQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCV SPKGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGM ARKPSRAYKI
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分子量
66 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
DNase II is an enzyme that plays a crucial role in the degradation of extracellular deoxyribonucleic acid (DNA) in various biological contexts, including cellular turnover and immune response. This endonuclease is particularly essential for the processing of apoptotic cells, as it facilitates the digestion of DNA released during programmed cell death, thereby preventing potential inflammatory responses and autoimmune diseases. Research into recombinant DNase II has gained traction due to its therapeutic potential; for instance, recombinant forms of this enzyme are being explored for their utility in clearing nucleic acids from the bloodstream in conditions characterized by excessive cell death or during certain viral infections. Furthermore, understanding the structural and functional properties of DNase II can provide insights into its mechanism of action and its interaction with substrates, paving the way for the development of enzyme inhibitors or activators that could be beneficial in treating diseases associated with aberrant DNA accumulation. Several studies have focused on the cloning, expression, and purification of DNase II, employing various expression systems to produce active recombinant proteins for functional assays. As our understanding of the enzyme’s role in both health and disease deepens, DNase II continues to be a promising target in the field of biomedicine, with ongoing research aimed at unlocking its full therapeutic potential.
