| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | DDX55; KIAA1595; ATP-dependent RNA helicase DDX55; DEAD box protein 55 |
| Entrez GeneID | 57696; |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human DDX55. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与DDX55抗体相关的模拟参考文献示例(请注意此为基于领域知识的虚构示例，建议通过PubMed/Google Scholar查询真实文献)：
1. **文献名称**: DDX55 as a novel biomarker in hepatocellular carcinoma: Antibody development and clinical validation
**作者**: Li X, et al.
**摘要**: 开发了针对DDX55蛋白的小鼠单克隆抗体，通过免疫组化证实DDX55在肝癌组织中高表达，且与患者生存率负相关，提示其作为肝癌诊断标志物的潜力。
2. **文献名称**: Functional characterization of DDX55 in viral RNA sensing using specific neutralizing antibodies
**作者**: Garcia-Ruiz C, et al.
**摘要**: 利用兔多克隆抗体阻断DDX55蛋白，发现其参与宿主细胞对RNA病毒(如流感病毒)的先天免疫应答，揭示了DDX55在抗病毒信号通路中的调控作用。
3. **文献名称**: A high-affinity humanized DDX55 antibody for targeted degradation in prostate cancer cells
**作者**: Watanabe T, et al.
**摘要**: 报道了人源化DDX55抗体偶联PROTAC分子的开发，在体外实验中成功降解前列腺癌细胞中的DDX55蛋白，并显著抑制肿瘤细胞增殖。
建议通过以下关键词检索真实文献：
`DDX55 antibody` `"DDX55" AND "antibody"` `DEAD-box helicase 55`
数据库推荐：PubMed (https://pubmed.ncbi.nlm.nih.gov) / Google Scholar
**Background of DDX55 Antibody**
DDX55 (DEAD-box helicase 55) is a member of the DEAD-box RNA helicase family, characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. These enzymes play critical roles in RNA metabolism, including unwinding RNA secondary structures, regulating transcription, splicing, RNA transport, and ribosome biogenesis. DDX55 is primarily localized to the nucleolus and is implicated in cell cycle progression, proliferation, and viral defense mechanisms. Dysregulation of DDX55 has been linked to cancers, where its overexpression correlates with tumor growth and poor prognosis.
Antibodies targeting DDX55 are essential tools for studying its expression, localization, and functional roles. These antibodies are typically developed in immunized hosts (e.g., rabbits or mice) using immunogenic peptides or recombinant protein fragments specific to DDX55. Validated applications include Western blotting, immunofluorescence, immunohistochemistry, and co-immunoprecipitation. High-quality DDX55 antibodies exhibit specificity confirmed by knockout controls or siRNA knockdown, ensuring minimal cross-reactivity with other DEAD-box proteins.
Research utilizing DDX55 antibodies has advanced understanding of its involvement in ribosome assembly, cell cycle checkpoints, and interactions with viral RNAs or proteins. Such studies are critical for exploring DDX55 as a potential biomarker or therapeutic target in cancer and infectious diseases. Commercial DDX55 antibodies often come with validation data, ensuring reproducibility in experimental models ranging from cell lines to clinical tissue samples.
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