| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALDH1A3 |
| Uniprot No | P47895 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-512aa |
| 氨基酸序列 | ATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP |
| 预测分子量 | 58.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ALDH1A3重组蛋白的模拟参考文献及摘要概括(虚构内容，供参考)：
1. **"ALDH1A3 recombinant protein promotes stemness in glioblastoma via retinoic acid signaling"**
- 作者：Chen L, et al.
- 摘要：研究通过大肠杆菌表达系统纯化ALDH1A3重组蛋白，证实其在胶质母细胞瘤细胞中通过代谢视黄酸增强肿瘤干细胞自我更新能力。
2. **"Structural and functional characterization of human ALDH1A3 recombinant enzyme"**
- 作者：Singh R, et al.
- 摘要：解析ALDH1A3重组蛋白的晶体结构，揭示其底物结合口袋的关键氨基酸残基，并验证其对乙醛和视黄醛的高催化效率。
3. **"ALDH1A3 as a therapeutic target in cancer: Insights from recombinant protein-based inhibitor screening"**
- 作者：Wang Y, et al.
- 摘要：利用ALDH1A3重组蛋白建立高通量抑制剂筛选平台，发现小分子化合物DEAB类似物可特异性抑制其酶活，抑制乳腺癌转移。
(注：以上为模拟内容，实际文献需通过PubMed/Google Scholar检索关键词如"ALDH1A3 recombinant protein"或结合具体研究领域筛选。)
ALDH1A3 (Aldehyde Dehydrogenase 1 Family Member A3) is a member of the aldehyde dehydrogenase superfamily, which plays a critical role in cellular detoxification by oxidizing endogenous and exogenous aldehydes to their corresponding carboxylic acids. This enzyme is particularly notable for its involvement in retinoic acid (RA) biosynthesis, a key signaling molecule regulating cell differentiation, proliferation, and apoptosis. ALDH1A3 is highly expressed in stem cells and cancer stem cells (CSCs), where it contributes to maintaining self-renewal capacity and resistance to chemotherapeutic agents. Its overexpression has been linked to poor prognosis in various cancers, including glioblastoma, breast cancer, and melanoma, making it a potential therapeutic target and biomarker.
Recombinant ALDH1A3 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional and structural studies. The purified protein retains enzymatic activity, requiring NAD+ as a cofactor for catalyzing aldehyde oxidation. Researchers utilize this recombinant form to investigate substrate specificity, inhibitor screening, and mechanistic insights into ALDH1A3's role in cancer biology and stem cell regulation. Additionally, it aids in developing assays to identify small-molecule inhibitors that could suppress CSC-associated chemoresistance or tumor recurrence.
The protein's structural features, including its conserved catalytic domain and oligomerization properties, are also explored using recombinant versions. Studies leveraging ALDH1A3 recombinant protein have advanced our understanding of its tissue-specific functions, interactions with signaling pathways (e.g., Wnt/β-catenin), and potential applications in regenerative medicine. Overall, ALDH1A3 recombinant tools are vital for dissecting its biological roles and translational relevance in disease.
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