| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALB1 |
| Uniprot No | P05937 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-261aa |
| 氨基酸序列 | AESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN |
| 预测分子量 | 30.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CALB1重组蛋白** 的3-4条参考文献示例，包含文献名称、作者及摘要概括：
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1. **《Expression and Purification of Recombinant Human Calbindin-D28k (CALB1) in Escherichia coli》**
- **作者**: Smith A, et al.
- **摘要**: 研究报道了在大肠杆菌中高效表达CALB1重组蛋白的优化方法，通过融合标签(如His-Tag)简化纯化流程，并验证了重组蛋白的钙结合活性，为后续功能研究提供基础工具。
2. **《Structural Insights into CALB1 by X-ray Crystallography: Implications for Calcium Signaling》**
- **作者**: Lee JH, Kim S.
- **摘要**: 利用X射线晶体学解析了CALB1重组蛋白的三维结构，揭示了其EF-hand结构域与钙离子的结合模式，探讨了其在神经元钙缓冲及信号传导中的潜在机制。
3. **《CALB1 Recombinant Protein Attenuates Neurodegeneration in a Parkinson’s Disease Model》**
- **作者**: Zhang Y, et al.
- **摘要**: 在帕金森病细胞模型中，外源性添加CALB1重组蛋白显著减少了多巴胺能神经元的钙超载和凋亡，提示其作为神经保护剂的治疗潜力。
4. **《Development of a CALB1-Based Biosensor for Calcium Imaging in Live Cells》**
- **作者**: Johnson R, Patel K.
- **摘要**: 将CALB1重组蛋白与荧光蛋白融合，开发了一种新型钙离子生物传感器，可实时监测活细胞内的钙动态变化，灵敏度优于传统染料。
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**注**：以上文献为示例性质，实际引用时建议通过PubMed、Web of Science等平台检索具体论文，并核对作者及发表年份。
CALB1 (Calbindin-D28k) is a calcium-binding protein belonging to the EF-hand superfamily, first identified for its role in intestinal calcium absorption. It is widely expressed in vertebrates, notably in the brain, kidneys, and endocrine tissues. Structurally, CALB1 contains six EF-hand domains, though only four actively bind calcium ions, enabling it to buffer intracellular calcium levels and modulate calcium-dependent signaling pathways. Its ability to regulate calcium homeostasis is critical for neuronal function, protecting cells from calcium-induced excitotoxicity, and supporting synaptic plasticity. Dysregulation of CALB1 has been linked to neurodegenerative disorders, such as Alzheimer’s and Parkinson’s diseases, highlighting its neuroprotective significance.
Recombinant CALB1 protein is engineered using expression systems like *E. coli*, yeast, or mammalian cells to produce purified, functional protein for research and therapeutic applications. This recombinant form retains calcium-binding activity and structural stability, making it a valuable tool for studying calcium signaling mechanisms, neuronal survival, and disease pathology. In drug development, it serves as a reference protein for screening calcium pathway-targeting compounds. Additionally, CALB1’s role as a biomarker in certain cancers (e.g., colorectal, breast) has spurred interest in recombinant variants for diagnostic assays. Advances in protein engineering have improved yield and solubility, facilitating large-scale studies. However, challenges remain in mimicking post-translational modifications critical for its native interactions. Ongoing research explores its therapeutic potential, including gene therapy for calcium-related disorders. Overall, recombinant CALB1 bridges foundational insights into calcium biology with translational innovations in neurology and oncology.
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