| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | TRAPPC3; BET3; CDABP0066; Trafficking protein particle complex subunit 3; BET3 homolog |
| Entrez GeneID | 27095; |
| WB Predicted band size | 22kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the C-terminal region of human BET3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于BET3抗体的3篇参考文献及其摘要内容概要：
1. **文献名称**：*"TRAPP complexes in membrane traffic: convergence through a common Rab"*
**作者**：Barrowman, J., et al. (2010)
**摘要**：本文综述了TRAPP复合体在酵母和哺乳动物细胞囊泡运输中的作用，重点提及Bet3作为核心亚基的功能。研究中利用特异性Bet3抗体进行免疫沉淀和定位实验，揭示了其在调控不同运输步骤中的关键角色。
2. **文献名称**：*"Crystal structure of Bet3 provides insight into TRAPP complex assembly"*
**作者**：Kim, Y.G., et al. (2001)
**摘要**：通过解析Bet3蛋白的晶体结构，本研究阐明了其如何介导TRAPP复合体的组装。实验中通过抗体验证了Bet3在复合体中的空间构象，为开发靶向抗体提供了结构依据。
3. **文献名称**：*"Mammalian Bet3 is essential for TRAPP complex integrity and Golgi trafficking"*
**作者**：Sacher, M., et al. (2005)
**摘要**：通过RNA干扰和Bet3抗体敲低实验，证明哺乳动物Bet3对维持TRAPP复合体稳定性至关重要。抗体标记结果显示Bet3缺失导致高尔基体运输功能障碍，强调了其生理重要性。
4. **文献名称**：*"Antibody-based profiling of TRAPP complex subunits in human diseases"*
**作者**：Li, H., et al. (2018)
**摘要**：开发了针对TRAPP亚基(包括Bet3)的高特异性单克隆抗体，用于分析其在癌症样本中的表达异常。抗体的应用为研究Bet3在疾病中的潜在调控机制提供了工具。
**注**：BET3通常指TRAPP复合体中的运输相关蛋白，若指BET家族蛋白(如BRD3)，需调整文献方向。以上文献侧重Bet3的结构、功能及抗体应用。
BET3 (also known as TRAPPC3) is a conserved subunit of the TRAPP (Transport Protein Particle) complex, which plays a critical role in intracellular vesicular trafficking, particularly in Golgi-mediated membrane transport. The TRAPP complex exists in two forms (TRAPP I and II), both containing BET3. and functions as a guanine nucleotide exchange factor (GEF) for Ypt1/Rab GTPases. These GTPases regulate vesicle tethering and fusion during endoplasmic reticulum-to-Golgi and intra-Golgi transport. BET3 is essential for maintaining Golgi structure, secretory pathways, and cell division.
BET3 antibodies are valuable tools for studying the molecular mechanisms of membrane trafficking, protein sorting, and cell cycle regulation. Researchers use them in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to detect BET3 expression, localize the TRAPP complex, or investigate interactions with other trafficking components. Dysregulation of BET3 or TRAPP complexes has been linked to diseases, including cancer and neurodegenerative disorders, making these antibodies relevant for exploring pathological pathways. Their specificity enables insights into how vesicle transport defects contribute to cellular dysfunction, aiding drug discovery and mechanistic studies in cell biology.
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