| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE1B |
| Uniprot No | Q01064 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAVYIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD |
| 预测分子量 | 86 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PDE1B重组蛋白相关的代表性文献摘要信息(基于公开知识截止2021年10月)：
1. **文献名称**: "Expression and characterization of the human recombinant phosphodiesterase PDE1B in Escherichia coli"
**作者**: Bender AT et al.
**摘要**: 描述了人源PDE1B在大肠杆菌系统中的重组表达策略，通过His标签纯化获得活性酶，并验证其cAMP/cGMP水解活性及钙调蛋白依赖性调控特性。
2. **文献名称**: "Purification and regulation of a recombinant PDE1B2 isoform from Sf9 insect cells"
**作者**: Sonnenburg WK et al.
**摘要**: 报道在昆虫细胞中表达PDE1B2重组蛋白，揭示其钙/钙调蛋白激活机制，并发现该亚型对cGMP的特异性催化活性高于cAMP。
3. **文献名称**: "Structural insights into the regulatory mechanism of phosphodiesterase 1B by calcium/calmodulin"
**作者**: Liu S et al.
**摘要**: 通过X射线晶体学解析了重组人PDE1B与钙调蛋白复合物的三维结构，阐明了钙信号诱导的构象变化激活酶活性的分子机制。
*注：以上文献信息为示例性质，实际引用请通过PubMed/Google Scholar等学术数据库核实具体文献DOI及发表年份。*
PDE1B (phosphodiesterase 1B) is a calcium/calmodulin-dependent enzyme belonging to the phosphodiesterase superfamily, which hydrolyzes cyclic nucleotides cAMP and cGMP to regulate their intracellular concentrations. As a key modulator of cyclic nucleotide signaling, PDE1B plays critical roles in diverse physiological processes, including cardiovascular function, neuronal signaling, and immune responses. It is highly expressed in the brain, heart, and immune cells, where it fine-tunes cellular responses by balancing cyclic nucleotide levels. Dysregulation of PDE1B has been implicated in neurological disorders, cardiovascular diseases, and cancer, making it a potential therapeutic target.
Recombinant PDE1B protein is engineered through molecular cloning and heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to produce purified, functional enzyme for research. This recombinant form retains catalytic activity, allowing studies on its structure, kinetics, and inhibition. Researchers utilize it to investigate PDE1B’s substrate specificity, calcium/calmodulin dependency, and interactions with inhibitors or signaling partners. Its production often includes affinity tags (e.g., His-tag) for simplified purification and detection.
Applications span drug discovery, particularly in developing PDE1B-selective inhibitors for neurodegenerative diseases like Parkinson’s or Alzheimer’s, where cyclic nucleotide imbalances contribute to pathology. It also aids in studying immune cell regulation and cardiovascular disorders. Recombinant PDE1B enables high-throughput screening of compound libraries and mechanistic studies to elucidate its role in disease pathways. Additionally, it supports antibody development for diagnostic or therapeutic purposes. By providing a standardized, bioactive tool, recombinant PDE1B accelerates both basic research and translational studies targeting cyclic nucleotide signaling networks.
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