| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATF2 |
| Uniprot No | P39905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 76-211aa |
| 氨基酸序列 | KRSPDKQMAV LPRRERNRQA AAANPENSRG KGRRGQRGKN RGCVLTAIHL NVTDLGLGYE TKEELIFRYC SGSCDAAETT YDKILKNLSR NRRLVSDKVG QACCRPIAFD DDLSFLDDNL VYHILRKHSA KRCGCI |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATF2重组蛋白的3篇参考文献(示例为假设性文献，实际文献需根据具体研究补充)：
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1. **文献名称**: "Purification and functional analysis of recombinant ATF2 for DNA-binding studies"
**作者**: Smith J, et al.
**摘要**: 该研究描述了通过大肠杆菌系统表达并纯化重组人源ATF2蛋白，验证其与靶DNA序列(如CRE位点)的结合能力，并探究其磷酸化修饰对DNA结合活性的调控作用。
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2. **文献名称**: "ATF2 recombinant protein enhances stress-induced apoptosis in cancer cells"
**作者**: Lee H, et al.
**摘要**: 研究利用重组ATF2蛋白处理多种癌细胞系，发现其通过激活JNK/p38信号通路促进细胞凋亡，为靶向ATF2的癌症治疗策略提供了实验依据。
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3. **文献名称**: "Structural characterization of ATF2 dimerization domain by X-ray crystallography"
**作者**: Tanaka K, et al.
**摘要**: 通过重组表达ATF2的亮氨酸拉链二聚化结构域并进行X射线晶体学分析，揭示了其与其他bZIP转录因子(如c-Jun)相互作用的分子机制。
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(注：以上文献为示例，实际引用需根据具体研究替换为真实发表的论文。)如需真实文献，可提供数据库检索关键词(如“ATF2 recombinant protein expression”)或具体研究方向进一步协助查询。
**Background of ATF2 Recombinant Protein**
Activating Transcription Factor 2 (ATF2), a member of the leucine zipper family of transcription factors, plays a critical role in regulating gene expression in response to cellular stress and extracellular signals. It contains a basic DNA-binding domain and a transactivation domain, enabling interactions with target gene promoters and co-regulators. ATF2 typically forms homodimers or heterodimers with other proteins, such as c-Jun, to modulate transcription. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation by stress-activated kinases (e.g., JNK, p38 MAPK), which enhance its transcriptional activity.
Recombinant ATF2 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cells, to produce purified, functional protein for research. The recombinant form often includes tags (e.g., GST, His-tag) for ease of purification and detection. Studies utilizing ATF2 recombinant protein focus on elucidating its DNA-binding properties, interaction partners, and regulatory mechanisms in pathways like apoptosis, inflammation, and DNA damage response.
ATF2 is implicated in diverse physiological and pathological processes, including cancer, neurodegeneration, and immune regulation. Dysregulation of ATF2 has been linked to tumor progression, where it can act as both an oncogene and tumor suppressor, depending on cellular context. Recombinant ATF2 serves as a vital tool for *in vitro* assays, structural studies, and drug discovery aimed at targeting ATF2-mediated pathways. Its application enhances understanding of stress-responsive gene networks and potential therapeutic strategies for diseases associated with ATF2 dysfunction.
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