| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/40000 | Human,Mouse,Rat |
| Aliases | HSD11B1; HSD11; HSD11L; Corticosteroid 11-beta-dehydrogenase isozyme 1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1 |
| Entrez GeneID | 3290; |
| WB Predicted band size | 35kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the N-terminal region of human 11β-HSD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于11β-HSD1抗体的3篇模拟参考文献示例(注：内容为模拟概括，非真实文献)：
1. **"Development and characterization of a monoclonal antibody specific for human 11β-hydroxysteroid dehydrogenase type 1"**
*Author: Smith A, et al.*
摘要：该研究开发了一种高特异性单克隆抗体，用于检测人组织中的11β-HSD1蛋白表达。通过免疫印迹和免疫组化验证了抗体在肝脏、脂肪组织中的特异性，为研究该酶的组织分布提供了工具。
2. **"11β-HSD1 expression in metabolic syndrome: Insights from antibody-based localization in murine models"**
*Author: Tanaka K, et al.*
摘要：利用定制多克隆抗体探究了代谢综合征小鼠模型中11β-HSD1的细胞定位，发现其在脂肪前体细胞和巨噬细胞中表达上调，提示其与炎症及糖皮质激素局部再活化的关联。
3. **"Selective inhibition of 11β-HSD1 by small molecules: Validation through antibody-mediated protein quantification"**
*Author: Patel R, et al.*
摘要：结合抗体介导的蛋白定量技术，验证了新型抑制剂对11β-HSD1酶活性的选择性抑制效果，表明该抗体可用于药物开发中靶点蛋白水平的动态监测。
11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) is a NADPH-dependent enzyme localized in the endoplasmic reticulum that catalyzes the conversion of inactive cortisone to active cortisol, a key glucocorticoid regulating metabolism, inflammation, and stress responses. It plays a critical role in tissue-specific glucocorticoid reactivation, particularly in liver, adipose tissue, and the central nervous system. Dysregulation of 11β-HSD1 has been implicated in metabolic disorders such as obesity, insulin resistance, and hypertension, making it a therapeutic target for metabolic syndrome.
Antibodies targeting 11β-HSD1 are essential tools for studying its expression, localization, and function in physiological and pathological contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research on glucocorticoid-mediated pathways. Specific 11β-HSD1 antibodies help distinguish it from the closely related isoform 11β-HSD2. which has opposing functions (inactivating cortisol). Validated antibodies ensure accurate quantification in tissues like adipose or liver, where its overexpression correlates with metabolic dysfunction.
Research using these antibodies has advanced understanding of local glucocorticoid amplification mechanisms and supported drug development, including 11β-HSD1 inhibitors for diabetes and obesity. Reliable antibodies remain crucial for exploring its role in aging, neurodegeneration, and stress-related disorders.
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