Rabbit Polyclonal Cleaved-Caspase-4p2(Q81) Antibody

Cleaved-Caspase-4 p2(Q81) antibody is a specialized tool used to detect the activated form of Caspase-4. a key protease involved in inflammatory signaling and pyroptosis. Caspase-4. part of the inflammatory caspase family (alongside Caspase-1. -5. and -11 in mice), is activated in response to intracellular lipopolysaccharide (LPS) during Gram-negative bacterial infections. Its cleavage into active fragments (p20/p10 or p22/p10. depending on species and context) enables its role in cleaving gasdermin D (GSDMD), triggering pore formation in cell membranes and the release of pro-inflammatory cytokines like IL-18 and IL-1β. The antibody specifically recognizes the p20/p22 subunit generated after proteolytic cleavage at aspartic acid residue 81 (Q81 in some nomenclature systems), serving as a marker for Caspase-4 activation. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or flow cytometry to study innate immune responses, bacterial pathogenesis, or inflammatory disorders. Its specificity helps distinguish active Caspase-4 from its inactive zymogen form, providing insights into mechanisms of pyroptosis, sepsis, or diseases linked to dysregulated inflammasome signaling.